Certolizumab Pegol antibody | AbD34136ia
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% milk, detection was performed using HRP conjugated Human Anti-Certolizumab pegol Antibody, clone AbD34136ia (HCA324) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with certolizumab pegol, human IgG1 kappa, human IgG1 lambda or Fab VH3-23 kappa1 (AbD18705) at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Certolizumab pegol Antibody, clone AbD34136ia (HCA324) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate. Data for certolizumab pegol are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with certolizumab pegol at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Certolizumab pegol Antibody, clone AbD34136ia (HCA324, black), clone AbD34201ia (HCA325, red) or clone AbD34202ia (HCA326, green). Detection was performed using HRP conjugated certolizumab pegol. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated over night with human TNFα at a concentration of 1 µg/ml. After washing and blocking with PBST+ 5% BSA, a pre-incubated mixture of biotinylated certolizumab pegol (0.3 µg/ml) plus increasing concentrations of Human Anti-Certolizumab Pegol Antibody, clone AbD34136ia (HCA324) was added. Free biotinylated certolizumab pegol, still capable of binding to the human TNFα coated plate, was detected using Streptavidin:HRP (STAR5B) and QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Certolizumab Pegol
Human Anti-Certolizumab pegol Antibody is a recombinant, inhibitory anti-idiotypic antibody in fully human IgG1 format for use in immunogenicity testing. It can be used as a positive control or reference standard in an ADA assay.
- Product Type
- Monoclonal Antibody
- Clone
- AbD34136ia
- Isotype
- IgG1
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA324 | E | 0.1 mg |
|
Human Anti-Certolizumab pegol Antibody, clone AbD34136ia, is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free humanized Fab fragment antibody certolizumab pegol. The antibody does not recognize human Tumor Necrosis Factor alpha (TNFα) or certolizumab pegol in complex with human TNFα.
Clone AbD34136ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 3 nM, and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA325 has affinity 1 nM and HCA326 has affinity 2 nM.
Certolizumab pegol (Cimzia) is a humanized Fab fragment antibody (50 kDa), covalently bound to two polyethylene glycol (PEG) chains (PEG2MAL, 40 kDa) via a thioether linkage; total molecular weight 90.8 kDa. PEG helps to delay the metabolism and elimination of the drug. The removal of the Fc region increases the half-life of the drug and prevents complement fixation and antibody-mediated cytotoxicity. The drug is approved for the treatment of Crohn's disease, rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. It binds to free and membrane-bound human TNFα and neutralizes its activity by inhibiting the binding of TNFα to human p55 and p75 TNF receptors.
View a summary of all anti-certolizumab pegol antibodies
Clone AbD34136ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 3 nM, and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA325 has affinity 1 nM and HCA326 has affinity 2 nM.
Certolizumab pegol (Cimzia) is a humanized Fab fragment antibody (50 kDa), covalently bound to two polyethylene glycol (PEG) chains (PEG2MAL, 40 kDa) via a thioether linkage; total molecular weight 90.8 kDa. PEG helps to delay the metabolism and elimination of the drug. The removal of the Fc region increases the half-life of the drug and prevents complement fixation and antibody-mediated cytotoxicity. The drug is approved for the treatment of Crohn's disease, rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. It binds to free and membrane-bound human TNFα and neutralizes its activity by inhibiting the binding of TNFα to human p55 and p75 TNF receptors.
View a summary of all anti-certolizumab pegol antibodies
Product Details
- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Certolizumab pegol
- Affinity
- The monovalent intrinsic affinity of AbD34136ia was measured as KD = 3 nM by real time, label free molecular interaction analysis on immobilized certolizumab pegol.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- Sold under license of U.S. Patents 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad.
Cimzia is a trademark of UCB Pharma S.A. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Certolizumab Pegol antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD34136ia can be used in an indirect ELISA. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.
Protocol: ADA bridging ELISA
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Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Certolizumab Pegol | HCA325 | E | 0.1 mg |
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| Human anti Certolizumab Pegol | HCA326 | E | 0.1 mg |
|
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| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody |
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