Certolizumab Pegol antibody | AbD34136ia

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Human anti Certolizumab Pegol

Human Anti-Certolizumab pegol Antibody is a recombinant, inhibitory anti-idiotypic antibody in fully human IgG1 format for use in immunogenicity testing. It can be used as a positive control or reference standard in an ADA assay.

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Human Anti-Certolizumab pegol Antibody, clone AbD34136ia, is a paratope specific, inhibitory anti-idiotypic antibody that specifically recognizes the free humanized Fab fragment antibody certolizumab pegol. The antibody does not recognize human Tumor Necrosis Factor alpha (TNFα) or certolizumab pegol in complex with human TNFα.

Clone AbD34136ia is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or reference standard in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 3 nM, and is one of three antibodies with different affinities offered for ADA assays. Antibody HCA325 has affinity 1 nM and HCA326 has affinity 2 nM.

Certolizumab pegol (Cimzia) is a humanized Fab fragment antibody (50 kDa), covalently bound to two polyethylene glycol (PEG) chains (PEG2MAL, 40 kDa) via a thioether linkage; total molecular weight 90.8 kDa. PEG helps to delay the metabolism and elimination of the drug. The removal of the Fc region increases the half-life of the drug and prevents complement fixation and antibody-mediated cytotoxicity. The drug is approved for the treatment of Crohn's disease, rheumatoid arthritis, psoriatic arthritis and ankylosing spondylitis. It binds to free and membrane-bound human TNFα and neutralizes its activity by inhibiting the binding of TNFα to human p55 and p75 TNF receptors.

View a summary of all anti-certolizumab pegol antibodies

Product Details

Product Form
Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01% Thiomersal
Certolizumab pegol
The monovalent intrinsic affinity of AbD34136ia was measured as KD = 3 nM by real time, label free molecular interaction analysis on immobilized certolizumab pegol.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Cimzia is a trademark of UCB Pharma S.A.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
For research purposes only

Applications of Certolizumab Pegol antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD34136ia can be used in an indirect ELISA. It can also be used as a surrogate positive control or calibrator in an anti-drug antibody assay.

Protocol: ADA bridging ELISA

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
Human anti Certolizumab Pegol HCA325 E 0.1 mg loader
Human anti Certolizumab Pegol HCA326 E 0.1 mg loader
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody

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