MHC Class II Monomorphic antibody | vpg3

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Mouse anti Cat MHC Class II Monomorphic

Product Type
Monoclonal Antibody
Clone
vpg3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA1348
Datasheet
C* F IP
SDS
2 ml

Mouse anti Cat MHC Class II antibody, clone vpg3 recognizes a monomorphic determinant on feline MHC class II molecules. MonomorphicThe major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In cats, this is referred to as the feline leukocyte antigen (FLA) region.

Mouse anti Cat MHC Class II antibody, clone vpg3 recogniszes monomorphic feline MHC class II molecules which are expressed by antigen presenting cells, B cells, monocytes and both activated and resting T lymphocytes.

Product Details

Target Species
Cat
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 10mM HEPES and 5-10% foetal calf serum
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
IL-2 dependent feline T cells.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS0 mouse myeloma cell line

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of MHC Class II Monomorphic antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Immunohistology - Frozen 1
Immunohistology - Paraffin
Immunoprecipitation
  1. 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 50ul of the suggested working dilution to label 106 cells in 100ul

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for MHC Class II Monomorphic antibody

  1. Willett, B.J. et al. (1993) Infection with feline immunodeficiency virus is followed by the rapid expansion of a CD8+ lymphocyte subset.
    Immunology 78: 1-6.
  2. Willett, B.J., and Callanan, J.J. (1995) The expression of leucocyte differentiation antigens in the feline immune system., p 3-15. In Feline Immunology and Immunodeficiency (eds.) Willett, B.J. and Jarrett, O.
    Oxford University Press.
  3. Beatty, J.A. et al. (1996) A longitudinal study of feline immunodeficiency virus-specific cytotoxic T lymphocytes in experimentally infected cats, using antigen-specific induction.
    J Virol. 70 (9): 6199-206.
  4. Avery, P.R. et al. (2007) Sustained generation of tissue dendritic cells from cats using organ stromal cell cultures.
    Vet Immunol Immunopathol. 117 (3-4): 222-35.
  5. Mumaw, J.L. et al. (2015) Feline mesenchymal stem cells and supernatant inhibit reactive oxygen species production in cultured feline neutrophils.
    Res Vet Sci. 103: 60-9.
  6. Hein, A. et al. (2003) Ramified feline microglia selects for distinct variants of feline immunodeficiency virus during early central nervous system infection.
    J Neurovirol. 9 (4): 465-76.

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