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CD5 antibody | FE1.1B11

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Anti Cat CD5 Antibody, clone FE1.1B11 thumbnail image 1
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Published customer image:
Mouse anti Cat CD5 antibody, clone FE1.1B11 (MCA2038S) used for the immunophenotyping of feline lymphoma samples by flow cytometry.
Image caption:
Representative plots of immunophenotyping by flow cytometry. (A) FNA of an intestinal mass, alimentary B‐cell lymphoma, CD4− (0%), CD5− (0%), CD8α/β− (0%), CD21+ (95%) (cat #1). (B) FNA of a peripheral lymph node, multicentric lymphoma, CD4+ (31%), CD5+ (53%), CD8α/β+ (15%), CD21+ (32%) (failure in detection of a predominant antigenically homogeneous lymphoid population) (cat #10). (C) FNA of a peripheral lymph node, reactive lymphoid hyperplasia, CD4+ (21%), CD5+ (53%), CD8α/β+ (26%), CD21+ (44%) (cat #16). *Red line (isotype control), green line (test sample).

From: Guzera M, Cian F, Leo C, Winnicka A, Archer J.
The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
Vet Comp Oncol. 2016 Aug;14 Suppl 1:40-51.
doi: 10.1111/vco.12098.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

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Mouse anti Cat CD5:FITC

Product Type
Monoclonal Antibody
Clone
FE1.1B11
Isotype
IgG1
Specificity
CD5

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
MCA2038F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader
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Mouse anti Cat CD5 antibody, clone FE1.1B11 recognizes feline CD5, a 67kDa cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.

Target Species
Cat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD5 antibody

  1. Roccabianca, P. et al. (2000) Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine.
    Vet Immunol Immunopathol. 75 (1-2): 27-42.
  2. Riondato, F. et al. (2003) Effects of interferon alpha (INF-alpha) therapy on peripheral blood lymphocyte subsets from FIV and FeLV naturally infected cats.
    Vet Res Commun. 27: 429-32.
  3. Affolter, V.K. and Moore, P.F. (2006) Feline progressive histiocytosis.
    Vet Pathol. 43: 646-55.
  4. Roccabianca, P. et al. (2006) Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia: primary intestinal origin with predominance of a CD3/CD8(alpha)(alpha) phenotype.
    Vet Pathol. 43: 15-28.
  5. Abkowitz, J.L. et al. (2009) In utero transplantation of monocytic cells in cats with alpha-mannosidosis.
    Transplantation. 88: 323-9.
  6. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.

RRID
AB_323962
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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