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CD5 antibody | FE1.1B11

Mouse anti Cat CD5:FITC

Product Type
Monoclonal Antibody
Clone
FE1.1B11
Isotype
IgG1
Specificity
CD5

Product Code Applications Pack Size List Price Your Price Qty
MCA2038F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Cat CD5 antibody, clone FE1.1B11 recognizes feline CD5, a 67kDa cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.

Target Species
Cat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
>
List Price Your Price
loader
Description Mouse IgG1 Negative Control:FITC

References for CD5 antibody

  1. Roccabianca, P. et al. (2000) Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine.
    Vet Immunol Immunopathol. 75 (1-2): 27-42.
  2. Riondato, F. et al. (2003) Effects of interferon alpha (INF-alpha) therapy on peripheral blood lymphocyte subsets from FIV and FeLV naturally infected cats.
    Vet Res Commun. 27: 429-32.
  3. Affolter, V.K. and Moore, P.F. (2006) Feline progressive histiocytosis.
    Vet Pathol. 43: 646-55.
  4. Roccabianca, P. et al. (2006) Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia: primary intestinal origin with predominance of a CD3/CD8(alpha)(alpha) phenotype.
    Vet Pathol. 43: 15-28.
  5. Abkowitz, J.L. et al. (2009) In utero transplantation of monocytic cells in cats with alpha-mannosidosis.
    Transplantation. 88: 323-9.
  6. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.
  7. Rütgen, B.C. et al. (2022) Composition of lymphocyte subpopulations in normal and mildly reactive peripheral lymph nodes in cats.
    J Feline Med Surg. 24 (2): 77-90.
  8. Cha, S. et al. (2023) Non-B, Non-T Acute Lymphoblastic Leukemia in a Cat
    Journal of Veterinary Clinics. 40 (4): 298-302.
  9. View The Latest Product References
  10. Rütgen, B.C. et al. (2024) Flowcytometric data of intermediate-large cell gastrointestinal lymphoma presenting a gross mass in 32 cats - "let them glow in the flow".
    Front Vet Sci. 11: 1378826.
  11. Wolfesberger, B. et al. (2024) Immunophenotype investigation in feline intestinal non-B-cell lymphoma.
    J Comp Pathol. 212: 20-26.

RRID
AB_323962

MCA2038F

151122 166368 1807

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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