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CD5 antibody | FE1.1B11

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Anti Cat CD5 Antibody, clone FE1.1B11 gallery image 1
Published customer image:
Mouse anti Cat CD5 antibody, clone FE1.1B11 (MCA2038S) used for the immunophenotyping of feline lymphoma samples by flow cytometry.
Image caption:
Representative plots of immunophenotyping by flow cytometry. (A) FNA of an intestinal mass, alimentary B‐cell lymphoma, CD4− (0%), CD5− (0%), CD8α/β− (0%), CD21+ (95%) (cat #1). (B) FNA of a peripheral lymph node, multicentric lymphoma, CD4+ (31%), CD5+ (53%), CD8α/β+ (15%), CD21+ (32%) (failure in detection of a predominant antigenically homogeneous lymphoid population) (cat #10). (C) FNA of a peripheral lymph node, reactive lymphoid hyperplasia, CD4+ (21%), CD5+ (53%), CD8α/β+ (26%), CD21+ (44%) (cat #16). *Red line (isotype control), green line (test sample).

From: Guzera M, Cian F, Leo C, Winnicka A, Archer J.
The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
Vet Comp Oncol. 2016 Aug;14 Suppl 1:40-51.
doi: 10.1111/vco.12098.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Cat CD5:FITC

Mouse anti Cat CD5:RPE

Mouse anti Cat CD5

Product Type
Monoclonal Antibody
Clone
FE1.1B11
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2038F
Datasheet
F
SDS
0.1 mg loader

MCA2038PE
Datasheet
F
SDS
100 Tests loader

MCA2038S
Datasheet
C F
SDS
2 ml loader

Mouse anti Cat CD5 antibody, clone FE1.1B11 recognizes feline CD5, a 67kDa cell surface glycoprotein expressed by peripheral T lymphocytes and thymocytes.

Product Details

Target Species
Cat
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Tissue Culture Supernatant - liquid
Reconstitution
Reconstitute with 1 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

This product should be stored undiluted.

DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

Regulatory
For research purposes only

Applications of CD5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:FITC MCA1209F F 0.1 mg loader
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA1209PE F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
Mouse IgG1 Negative Control MCA1209 F 0.1 mg loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Product Specific References

References for CD5 antibody

  1. Roccabianca, P. et al. (2000) Characterization of the diffuse mucosal associated lymphoid tissue of feline small intestine.
    Vet Immunol Immunopathol. 75 (1-2): 27-42.
  2. Riondato, F. et al. (2003) Effects of interferon alpha (INF-alpha) therapy on peripheral blood lymphocyte subsets from FIV and FeLV naturally infected cats.
    Vet Res Commun. 27: 429-32.
  3. Affolter, V.K. and Moore, P.F. (2006) Feline progressive histiocytosis.
    Vet Pathol. 43: 646-55.
  4. Roccabianca, P. et al. (2006) Feline large granular lymphocyte (LGL) lymphoma with secondary leukemia: primary intestinal origin with predominance of a CD3/CD8(alpha)(alpha) phenotype.
    Vet Pathol. 43: 15-28.
  5. Abkowitz, J.L. et al. (2009) In utero transplantation of monocytic cells in cats with alpha-mannosidosis.
    Transplantation. 88: 323-9.
  6. Guzera, M. et al. (2016) The use of flow cytometry for immunophenotyping lymphoproliferative disorders in cats: a retrospective study of 19 cases.
    Vet Comp Oncol. 14 Suppl 1: 40-51.

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