Browsing Modes for Business Users

Canakinumab antibody | AbD54116ia

Human anti Canakinumab

Product Type
Monoclonal Antibody
Clone
AbD54116ia
Isotype
IgG1
Specificity
Canakinumab

Product Code Applications Pack Size List Price Your Price Qty
New HCA419
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Human Anti-Canakinumab Antibody, clone AbD54116ia is a paratope specific, inhibitory, anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug canakinumab, and its biosimilar products. It does not recognize the drug target, interleukin-1 beta (IL-1β), nor canakinumab in complex with IL-1β. This antibody can be used to measure free canakinumab levels in serum from patients.

HCA419 is a fully human recombinant monoclonal antibody with IgG1 isotype and is suitable as a surrogate positive control or calibrator in an anti-drug antibody (ADA) assay. This antibody has a monovalent intrinsic affinity of 9.6nM and is one of the three antibodies offered for ADA assays. Antibody HCA417 has an affinity of 1.5nM and HCA418 has an affinity of 7.7nM.

Canakinumab (trade name Ilaris) is a fully humanized monoclonal IgG1/kappa antibody with allotype G1m3. It binds to interleukin-1 beta (IL-1β) to neutralize up-regulated 1β signaling, resulting in suppression of inflammation in patients with disorders of autoimmune origin. Canakinumab is indicated for treatment of Cryopyrin-Associated Periodic Syndromes (CAPS), three rare and serious auto-inflammatory diseases, Still’s disease, and gouty arthritis.

Product Form
Human IgG1 antibody (lambda light chain) selected from the HuCAL phage display library and expressed in a human cell line. This product is supplied as a liquid.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Source
HKB-11
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Canakinumab
Affinity
The intrinsic affinity of the monovalent form of Human Anti-Canakinumab Antibody, clone AbD54116ia is KD = 9.6 nM as measured by real time, label free molecular interaction analysis on immobilized canakinumab
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Ilaris is a trademark of Novartis AG.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
ELISA
Clone AbD54116ia can be used in indirect ELISA to detect canakinumab. It can also be used as a surrogate positive control or a calibrator in an anti-drug antibody (ADA) assay, together with HCA417 and HCA418

Protocol: ADA bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
Hispec Assay Diluent BUF049A E IY 50 ml
List Price Your Price
Description Hispec Assay Diluent
Human anti Canakinumab HCA417 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
Human anti Canakinumab HCA418 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
LYNX Rapid HRP Antibody Conjugation Kit LNK002P CJ 3 Conjugations For 400µg Antibody
List Price Your Price
Description LYNX Rapid HRP Antibody Conjugation Kit
Human anti Canakinumab TZA063 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab
Human anti Canakinumab TZA064 E 0.1 mg loader
List Price Your Price
loader
Description Human anti Canakinumab

Synonyms
Ilaris

HCA419

169857

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with Canakinumab specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

Please note