Belatacept antibody | AbD36935ia
A microtiter plate was coated overnight with recombinant human CD80-Fc at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of Belatacept-HRP (0.15 µg/ml) spiked with increasing concentrations of Human Anti-Belatacept Antibody, clone AbD36935ia (HCA393) in monovalent Fab format was added. Free Belatacept-HRP, still capable of binding to the human CD80-Fc coated plate, was detected by QuantaBlu™ fluorogenic peroxidase substrate. Data are shown as the mean of three measurements. HRP conjugation of belatacept was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with Human Anti-Belatacept Antibody, clone AbD37060 (HCA391) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of belatacept. Detection was performed using HRP conjugated Human Anti-Belatacept Antibody, clone AbD36935ia (HCA393) at a concentration of 2 µg/mL in HISPEC assay diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belatacept at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, PBST with 10% human serum was added spiked with increasing concentrations of Human Anti-Belatacept Antibody, clone AbD37060ia (HCA392, black), clone AbD36935ia (HCA393, blue) or clone AbD37065ia (HCA336, red). Detection was performed using HRP conjugated belatacept at a concentration of 2 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P). Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Belatacept Antibody, clone AbD36935ia (HCA393) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
A microtiter plate was coated overnight with belatacept, abatacept, human IgG1 kappa control or human Fc-tag (same allotype as belatacept and abatacept) at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using HRP conjugated Human Anti-Belatacept Antibody, clone AbD36935ia (HCA393) titrated to the given concentrations in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements. HRP conjugation of the antibody used in this assay was performed using a LYNX Rapid HRP Antibody Conjugation Kit (LNK002P).
Human anti Belatacept
- Product Type
- Monoclonal Antibody
- Clone
- AbD36935ia
- Isotype
- IgG1
- Specificity
- Belatacept
Antibody Quality and Performance Guarantee
| Human Anti-Belatacept Antibody, clone AbD36935ia is an affinity-matured, non-inhibitory, anti-idiotypic antibody. It recognizes the drugs belatacept and abatacept, and their biosimilar products as well as cytotoxic T-lymphocyte antigen-4 (CTLA-4), also known as CD152. The antibody binds to free belatacept, abatacept and human CTLA4 and can be used to measure free belatacept, abatacept or their biosimilars in patient samples.
Clone 36935ia is a monoclonal antibody with hIgG1 isotype and is suitable as a positive control or as detection antibody for Belatacept in a PK bridging ELISA. This Anti-Belatacept Antibody cross-reacts with the drug abatacept (Orencia). It is only 2 amino acids different from abatacept. LEA29Y-belatacept was constructed by two amino acid substitutions (L104E and A29Y) compared to abatacept that gave rise to slower dissociation rates for both CD86 and CD80. Belatacept (Nulojix) is a genetically engineered fusion protein which consists of the functional binding domain of modified human CTLA-4 and the Fc domain of human monoclonal immunoglobulin of the IgG1 subclass. The Fc part has the allotype IGHG1*05. Belatacept is indicated for prophylaxis of organ rejection in adult patients receiving a kidney transplant. It is to be used in combination with basiliximab induction, mycophenolate mofetil, and corticosteroids. View a summary of all anti-belatacept antibodies |
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- Product Form
- Human IgG1 antibody (kappa light chain) selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Belatacept
- Affinity
- The intrinsic affinity of the monovalent form of Human anti Belatacept antibody, clone AbD36935ia is KD = 6 nM as measured by real time, label free molecular interaction analysis on immobilized Belatacept..
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Nulojix is a trademark of Bristol-Myers Squibb Company. - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- Human anti Belatacept antibody, clone AbD36935ia can be used in a direct or indirect ELISA. It can also be used as the detection antibody for belatacept in a PK bridging ELISA together with HCA391 as the capture reagent. Moreover, HCA393 may be used as a calibrator for the set-up of an anti-drug antibody (ADA) assay.
PK bridging ELISA
ADA bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Belatacept | HCA391 | E | 0.1 mg |
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| Description | Human anti Belatacept | ||||||
| Human anti Belatacept | HCA392 | E | 0.1 mg |
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| Description | Human anti Belatacept | ||||||
| LYNX Rapid HRP Antibody Conjugation Kit | LNK002P | CJ | 3 Conjugations For 400µg Antibody | Log in | |||
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| Description | LYNX Rapid HRP Antibody Conjugation Kit | ||||||
- Synonyms
- Nulojix
- RRID
- AB_2943073
HCA393
165897
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with Belatacept specificity
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