Avelumab antibody | AbD40521ad
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Avelumab Antibody, clone AbD40521ad (TZA041) at a concentration of 2 μg/ml followed by an HRP-labelled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with avelumab, recombinant His-tagged human PD-L1, adalimumab, human IgG1 kappa control and human IgG1 lambda control at a concentration of 5 µg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Avelumab Antibody, clone AbD40521ad (TZA041) titrated to the given concentrations in PBST followed by an HRP labeled anti-DYKDDDDK tag antibody in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with recombinant His-tagged human PD-L1 at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a pre-incubated mixture of avelumab (0.15 µg/ml) plus increasing concentrations of Human Anti-Avelumab Antibody, clone AbD40521ad (TZA041) or Type 3 clone AbD42040ad (TZA042), both in the monovalent Fab format, were added. Free avelumab, still capable of binding to the human PD-L1 coated plate, was detected using HRP conjugated Mouse Anti-Human IgG (Fc) CH2 Domain Antibody (MCA647P) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
A microtiter plate was coated overnight with Human Anti-Avelumab Antibody, clone AbD40521ad (TZA041) at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of avelumab. Detection was performed using HRP conjugated Human Anti-Avelumab Antibody, clone AbD40519pap (TZA040P) at a concentration of 0.5 µg/ml in HISPEC Assay Diluent (BUF049A) followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
Human anti Avelumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD40521ad
- Isotype
- Fab antibody
- Specificity
- Avelumab
Antibody Quality and Performance Guarantee
TZA041
| Human Anti-Avelumab Antibody, clone AbD40521ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug avelumab. The antibody does not recognize free PD-L1 nor avelumab in complex with PD-L1. This antibody can be used to measure free avelumab levels in serum from patients. TZA041 is available in monovalent Fab unconjugated format. It can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free avelumab captured via immobilized PD-L1. Anti-Avelumab monovalent Fab antibody TZA041 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog. View a summary of all SpyCatcher products Avelumab (Bavencio) is a human IgG1/lambda antibody with allotype IGHG1*05 (G1m17,1). It binds to programmed death-ligand 1 (PD-L1, CD274) on tumor cells, blocking its interaction with PD-1 and B7-1 receptors on T-lymphocytes. PD-L1 is an immune checkpoint protein expressed on tumor cells and tumor-infiltrating immune cells that down regulates T-cell function, so by blocking these receptors anti-tumor T-cell activity can be restored. In vitro, the drug has been shown to stimulate natural killer cell-mediated direct tumor cell lysis by inducing antibody-dependent cell-mediated cytotoxicity. Avelumab has been approved both as monotherapy for the treatment of adult patients with metastatic Merkel cell carcinoma (MCC) and for the treatment of advanced Renal Cell Carcinoma (RCC). |
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- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
- Preparation
- Metal chelate affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.0095% MIT
- Immunogen
- Avelumab
- Affinity
- The monovalent intrinsic affinity of AbD40521ad was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized avelumab.
- Approx. Protein Concentrations
- Antibody concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Bavencio is a registered trademark of Merck Serono (Pfizer as co-developer). - Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- Clone AbD40521ad can be used as the capture antibody for avelumab in a PK ELISA together with TZA040P as the detection antibody.
Protocol: PK bridging ELISA
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyTag3 Peptide | BLP086 | IY | 1 mg |
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| Description | SpyTag3 Peptide | ||||||
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Avelumab | HCA396 | E | 0.1 mg |
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| Description | Human anti Avelumab | ||||||
| Human anti Avelumab | HCA397 | E | 0.1 mg |
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| Description | Human anti Avelumab | ||||||
| Human anti Avelumab | HCA398 | E | 0.1 mg |
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| Description | Human anti Avelumab | ||||||
| Human anti Avelumab | TZA040 | E | 0.1 mg | Log in | |||
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| Description | Human anti Avelumab | ||||||
| Human anti Avelumab:HRP | TZA040P | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human anti Avelumab:HRP | ||||||
| Human anti Avelumab (Drug/Target Complex) | TZA042 | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human anti Avelumab (Drug/Target Complex) | ||||||
| Human anti Avelumab (Drug/Target Complex):HRP | TZA042P | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human anti Avelumab (Drug/Target Complex):HRP | ||||||
| BiSpyCatcher2:HRP | TZC002P | IY | 1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | BiSpyCatcher2:HRP | ||||||
- Synonyms
- Bavencio
TZA041
167372
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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