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Avelumab antibody | AbD40519

Human anti Avelumab

Product Type
Monoclonal Antibody
Fab antibody

Product Code Applications Pack Size List Price Your Price Qty
New TZA040
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price

Human Anti-Avelumab Antibody, clone AbD40519 is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug avelumab. The antibody does not recognize free PD-L1 nor avelumab in complex with PD-L1. This antibody can be used to measure free avelumab levels in serum from patients.

TZA040 is available in three formats: as a full length IgG1 antibody (HCA396) for use in ADA assays, as well as two formats incorporating a SpyTag: a monovalent recombinant Fab format (TZA040), and a bivalent SpyTag Fab format (TZA040P) coupled with a HRP conjugated BiSpyCatcher2 (TZC002P). Either Fab format can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free avelumab captured via immobilized PD-L1.

Anti-Avelumab monovalent Fab antibody TZA040 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Avelumab (Bavencio) is a human IgG1/lambda antibody with allotype IGHG1*05 (G1m17,1). It binds to programmed death-ligand 1 (PD-L1, CD274) on tumor cells, blocking its interaction with PD-1 and B7-1 receptors on T-lymphocytes. PD-L1 is an immune checkpoint protein expressed on tumor cells and tumor-infiltrating immune cells that down regulates T-cell function, so by blocking these receptors anti-tumor T-cell activity can be restored. In vitro, the drug has been shown to stimulate natural killer cell-mediated direct tumor cell lysis by inducing antibody-dependent cell-mediated cytotoxicity. Avelumab has been approved both as monotherapy for the treatment of adult patients with metastatic Merkel cell carcinoma (MCC) and for the treatment of advanced Renal Cell Carcinoma (RCC).
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Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
The monovalent intrinsic affinity of AbD40519ad was measured as KD = 0.9 nM by real time, label free molecular interaction analysis on immobilized avelumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
For research purposes only
12 months from date of despatch
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See for details.
His-tag is a registered trademark of EMD Biosciences.
Bavencio is a registered trademark of Merck Serono (Pfizer as co-developer).
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Clone AbD40519ad can be used as the detection antibody for avelumab in a PK ELISA together with TZA041 as the capture antibody.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
List Price Your Price
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Avelumab HCA396 E 0.1 mg loader
List Price Your Price
Description Human anti Avelumab
Human anti Avelumab HCA397 E 0.1 mg loader
List Price Your Price
Description Human anti Avelumab
Human anti Avelumab HCA398 E 0.1 mg loader
List Price Your Price
Description Human anti Avelumab
Human anti Avelumab:HRP TZA040P E 0.1 mg
List Price Your Price
Description Human anti Avelumab:HRP
Human anti Avelumab TZA041 E 0.1 mg
List Price Your Price
Description Human anti Avelumab
Human anti Avelumab (Drug/Target Complex) TZA042 E 0.1 mg
List Price Your Price
Description Human anti Avelumab (Drug/Target Complex)
Human anti Avelumab (Drug/Target Complex):HRP TZA042P E 0.1 mg
List Price Your Price
Description Human anti Avelumab (Drug/Target Complex):HRP
BiSpyCatcher2:HRP TZC002P IY 1 mg
List Price Your Price
Description BiSpyCatcher2:HRP




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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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