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Avelumab antibody | AbD40521ad

Human anti Avelumab

Product Type
Monoclonal Antibody
Clone
AbD40521ad
Isotype
Fab antibody
Specificity
Avelumab

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New TZA041
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SDS Safety Datasheet SDS
E 0.1 mg loader
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Human Anti-Avelumab Antibody, clone AbD40521ad is a paratope specific, inhibitory anti-idiotypic antibody (Type 1) that specifically recognizes the free human monoclonal antibody drug avelumab. The antibody does not recognize free PD-L1 nor avelumab in complex with PD-L1. This antibody can be used to measure free avelumab levels in serum from patients.

TZA041 is available in monovalent Fab unconjugated format. It can be used to develop a pharmacokinetic (PK) bridging ELISA to measure free avelumab captured via immobilized PD-L1.

Anti-Avelumab monovalent Fab antibody TZA041 has a SpyTag incorporated for conversion by the user into alternative antibody formats using any of the SpyCatchers available in our catalog.

View a summary of all SpyCatcher products

Avelumab (Bavencio) is a human IgG1/lambda antibody with allotype IGHG1*05 (G1m17,1). It binds to programmed death-ligand 1 (PD-L1, CD274) on tumor cells, blocking its interaction with PD-1 and B7-1 receptors on T-lymphocytes. PD-L1 is an immune checkpoint protein expressed on tumor cells and tumor-infiltrating immune cells that down regulates T-cell function, so by blocking these receptors anti-tumor T-cell activity can be restored. In vitro, the drug has been shown to stimulate natural killer cell-mediated direct tumor cell lysis by inducing antibody-dependent cell-mediated cytotoxicity. Avelumab has been approved both as monotherapy for the treatment of adult patients with metastatic Merkel cell carcinoma (MCC) and for the treatment of advanced Renal Cell Carcinoma (RCC).

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in a proprietary E. coli strain. The antibody is tagged with a DYKDDDDK tag, a SpyTag version 2 (VPTIVMVDAYKRYK) and a His6-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid.
Preparation
Metal chelate affinity chromatography
Source
E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.0095% MIT
Immunogen
Avelumab
Affinity
The monovalent intrinsic affinity of AbD40521ad was measured as KD = 1 nM by real time, label free molecular interaction analysis on immobilized avelumab.
Approx. Protein Concentrations
Antibody concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a registered trademark of EMD Biosciences.
Bavencio is a registered trademark of Merck Serono (Pfizer as co-developer).

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols for coupling a Fab antibody with a SpyTag to a SpyCatcher can be found at SpyTag and SpyCatcher Products, and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
Clone AbD40521ad can be used as the capture antibody for avelumab in a PK ELISA together with TZA040P as the detection antibody.

Protocol: PK bridging ELISA

Description Product Code Applications Pack Size List Price Your Price Quantity
SpyTag3 Peptide BLP086 IY 1 mg loader
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loader
Description SpyTag3 Peptide
Hispec Assay Diluent BUF049A E IY 50 ml
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Description Hispec Assay Diluent
Human anti Avelumab HCA396 E 0.1 mg loader
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loader
Description Human anti Avelumab
Human anti Avelumab HCA397 E 0.1 mg loader
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loader
Description Human anti Avelumab
Human anti Avelumab HCA398 E 0.1 mg loader
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loader
Description Human anti Avelumab
Human anti Avelumab TZA040 E 0.1 mg
List Price Your Price
Description Human anti Avelumab
Human anti Avelumab:HRP TZA040P E 0.1 mg
List Price Your Price
Description Human anti Avelumab:HRP
Human anti Avelumab (Drug/Target Complex) TZA042 E 0.1 mg
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Description Human anti Avelumab (Drug/Target Complex)
Human anti Avelumab (Drug/Target Complex):HRP TZA042P E 0.1 mg
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Description Human anti Avelumab (Drug/Target Complex):HRP
BiSpyCatcher2:HRP TZC002P IY 1 mg
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Description BiSpyCatcher2:HRP

Synonyms
Bavencio
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.

TZA041

167372

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