Alemtuzumab antibody | AbD16728
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 2 μg/ml followed by Mouse Anti-Strep-Tag Classic:HRP Antibody (MCA2489P) antibody in HISPEC Assay Dilutent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab. Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Filter by Application:
F E ResetHuman anti Alemtuzumab
- Product Type
- Monoclonal Antibody
- Clone
- AbD16728
- Isotype
- HuCAL Fab monovalent
- Specificity
- Alemtuzumab
Antibody Quality and Performance Guarantee
| Human anti Alemtuzumab antibody, clone AbD16728 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis. Human anti Alemtuzumab antibody, clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays. This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P). Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of View a summary of all anti-alemtuzumab antibodies. |
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library, expressed in E.coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography
- Source
- E.coli
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Alemtuzumab.
- Affinity
- The intrinsic affinity of the monovalent form of this antibody is KD=1.6 nM as measured by real time, label-free molecular interaction analysis on immobilized Alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA175P as the detection reagent. Protocol: PK bridging ELISA to measure free drug.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse anti V5-Tag | MCA1360 | C E F IF IP R WB | 1 mg |
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| Description | Mouse anti V5-Tag | ||||||
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
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| Description | Mouse anti V5-Tag:Biotin | ||||||
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
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| Description | Mouse anti V5-Tag:HRP | ||||||
| Mouse anti Strep-Tag Immo | MCA2488 | E | 0.1 mg |
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| Description | Mouse anti Strep-Tag Immo | ||||||
| Mouse anti Strep-Tag Classic | MCA2489 | E WB | 0.1 mg |
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| Description | Mouse anti Strep-Tag Classic | ||||||
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
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| Description | Mouse anti Strep-Tag Classic:HRP | ||||||
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
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| Hispec Assay Diluent | BUF049A | E IY | 50 ml | Log in | |||
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| Description | Hispec Assay Diluent | ||||||
| Human anti Alemtuzumab:HRP | HCA175P | E | 0.1 mg | Log in | |||
| List Price | Your Price | ||||||
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| Description | Human anti Alemtuzumab:HRP | ||||||
| Rat anti Human CD52 | MCA1642 | C CT E F IP P RE WB | 0.2 mg |
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| Description | Rat anti Human CD52 | ||||||
- RRID
- AB_11152765
- Licensed Use
- For in vitro. research purposes and for commercial applications for the provision of in vitro. testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
HCA174
1702
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