Alemtuzumab antibody | AbD16728
A microtiter plate was coated overnight with various antigens at a concentration of 5 μg/ml. After washing and blocking with PBST + 5% BSA, detection was performed using Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 2 μg/ml followed by Mouse Anti-Strep-Tag Classic:HRP Antibody (MCA2489P) antibody in HISPEC Assay Dilutent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
A microtiter plate was coated overnight with Human Anti-Alemtuzumab Antibody, clone AbD16728 (HCA174) at a concentration of 1 μg/ml. After washing and blocking with PBST + 5% BSA, 10% human serum was added spiked with increasing concentrations of alemtuzumab. Detection was performed using HRP conjugated Human Anti-Alemtuzumab Antibody, clone AbD16942_hIgG1 (HCA175P) at a concentration of 2 μg/ml in HISPEC Assay Diluent (BUF049A) and QuantaBlu Fluorogenic Peroxidase Substrate.
Human anti Alemtuzumab
Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of alemtuzumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA.
- Product Type
- Monoclonal Antibody
- Clone
- AbD16728
- Isotype
- HuCAL Fab monovalent
| Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|
| HCA174 | E | 0.1 mg |
|
Human anti Alemtuzumab antibody, clone AbD16728 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.
Human anti Alemtuzumab antibody, clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.
This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P).
Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding ofclone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.
View a summary of all anti-alemtuzumab antibodies.
Human anti Alemtuzumab antibody, clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.
This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P).
Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of
View a summary of all anti-alemtuzumab antibodies.
Product Details
- Product Form
- A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
- Preparation
- StrepTactin affinity chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- 0.01% Thiomersal
- Immunogen
- Alemtuzumab.
- Affinity
- The monovalent intrinsic affinity of this antibody was measured as KD=1.6 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
- Licensed Use
- For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
- Regulatory
- For research purposes only
Applications of Alemtuzumab antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA175P as the detection reagent. Protocol: PK bridging ELISA to measure free drug
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse anti V5-Tag | MCA1360 | C E F IF IP R WB | 1 mg |
|
|
| Mouse anti V5-Tag:Biotin | MCA1360B | C E WB | 0.1 mg |
|
|
| Mouse anti V5-Tag:HRP | MCA1360P | E WB | 0.1 mg |
|
|
| Mouse anti Strep-Tag Immo | MCA2488 | E | 0.1 mg |
|
|
| Mouse anti Strep-Tag Classic | MCA2489 | E WB | 0.1 mg |
|
|
| Mouse anti Strep-Tag Classic:HRP | MCA2489P | E WB | 75 µl |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Hispec Assay Diluent | BUF049A | E IY | 50 ml | ||
| Human anti Alemtuzumab:HRP | HCA175P | E | 0.1 mg |
|
|
| Rat anti Human CD52 | MCA1642 | C CT E F IP P RE WB | 0.2 mg |
|
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