Alemtuzumab antibody | AbD16728

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Human anti Alemtuzumab

Anti-alemtuzumab antibody is a recombinant, neutralizing anti-idiotypic antibody in monovalent Fab format; it is ideal for assay development to measure levels of alemtuzumab or biosimilars. It is recommended as a capture antibody in a PK bridging ELISA.

Product Type
Monoclonal Antibody
Clone
AbD16728
Isotype
HuCAL Fab monovalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA174 E 0.1 mg
Human anti Alemtuzumab antibody, clone AbD16728 recognizes alemtuzumab, a monoclonal antibody drug directed to human CD52 and used in the treatment of chronic lymphocytic leukemia (CLL), cutaneous T-cell lymphoma (CTCL) and T-cell lymphoma. Alemtuzumab is also used in some conditioning regimes for bone marrow transplantation, kidney transplantation and Islet cell transplantation along with clinical trial protocols for treatment of certain autoimmune diseases including multiple sclerosis.

Human anti Alemtuzumab antibody, clone AbD16728 is an anti-idiotypic antibody that specifically recognizes the alemtuzumab humanized monoclonal antibody and and can be used to measure the levels of alemtuzumab and biosimilar products using bioanalytical assays.

This antibody may be used in a bridging ELISA in conjunction with horseradish peroxidase conjugated clone AbD16942 (HCA175P).

Clone AbD16728 has been demonstrated to inhibit the binding of alemtuzumab to CD52-positive human cells in flow cytometry. It also blocks the binding of clone YTH34.5, the Rat monoclonal prototype antibody of alemtuzumab, to CD52+ cells.

View a summary of all anti-alemtuzumab antibodies.

Product Details

Product Form
A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL® phage display library, expressed in E. coli. The antibody is tagged with a V5 tag and a double extended Strep-tag. This antibody is supplied as a liquid.
Preparation
StrepTactin affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Alemtuzumab.
Affinity
The monovalent intrinsic affinity of this antibody was measured as KD=1.6 nM by real time, label-free molecular interaction analysis on immobilized alemtuzumab.
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use
For in vitro research purposes and for commercial applications for the provision of in vitro testing services to support preclinical and clinical drug development. Any re-sale in any form or any other commercial application needs a written agreement with Bio-Rad.
Regulatory
For research purposes only

Applications of Alemtuzumab antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
ELISA
This product may be used in an indirect ELISA or as a capture antibody in a sandwich ELISA together with HCA175P as the detection reagent. Protocol: PK bridging ELISA to measure free drug

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti V5-Tag MCA1360 1 mg C E F IF IP R WB
Mouse anti V5-Tag:Biotin MCA1360B 0.1 mg C E WB
Mouse anti V5-Tag:HRP MCA1360P 0.1 mg E WB
Mouse anti Strep-Tag Immo MCA2488 0.1 mg E
Mouse anti Strep-Tag Classic MCA2489 0.1 mg E WB
Mouse anti Strep-Tag Classic:HRP MCA2489P 75 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Hispec Assay Diluent BUF049A 50 ml E IY
Human anti Alemtuzumab:HRP HCA175P 0.1 mg E
Rat anti Human CD52 MCA1642 0.2 mg C CT E F IP P RE WB