Mitochondrial Permeability Transition:MitoPTTM JC-1 Kit

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Mitochondrial Permeability Transition:MitoPT<sup>TM</sup> JC-1 Kit gallery image 1

Jurkat cells were stained with MitoPTTM JC-1 and viewed through a fluorescence microscope using a broad band path filter. Non-apoptotic cells exhibit red stained mitochondria (2 cells at the top). Apoptotic cells at varying stages of mitochondrial membrane potential appear green (3 cells at the bottom)

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  • Mitochondrial Permeability Transition Kit
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  • Product Type
    Kits
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ICT943F, IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf100 Tests
    ICT943
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • The MitoPTTM JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      MitoPTTM JC-1 Reagent - lyophilized
      10X Assay Buffer, 30mL
      125uL vial 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP) concentrate in DMSO.
    • Preparing The Antibody
    • Test Principle
      An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPTTM JC-1 kit.

      The MitoPTTM JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
    • Buffer Solution
    • Storage
      Store the unopened kit (and each unopened component) at -20oC until the expiration date. Protect the MitoPTTM JC-1 reagent from light at all times.

      Once reconstituted, the 100X MitoPTTMJC-1 stock should be stored at -20oC protected from light.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
      MitoPTTM is a trademark of Immunochemistry Technologies, LLC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunofluorescence

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT943.pdf

    Additional Mitochondrial Permeability Transition:MitoPTTM JC-1 Kit Formats

    Formats Applications Sizes available
    Mitochondrial Permeability Transition:MitoPTTM JC-1 Kit : JC-1 Dye (Dual Green/Red Fluorescence) F, IF 100 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Mitochondrial Permeability Transition KitICT944400 TestsF, IF
          ICT944

          Recommended Positive Controls

            Histology Controls

              References

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