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Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit

Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit gallery image 1

Jurkat cells were stained with MitoPTTM JC-1 and viewed through a fluorescence microscope using a broad band path filter. Non-apoptotic cellsexhibit red stained mitochondria (2 cells at the top). Apoptotic cells at varying stages of mitochondrial membrane potential appear green (3 cells at the bottom)

  • Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit thumbnail image 1
  • Mitochondrial Permeability Transition Kit
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  • Product Type
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    ICT944F, IFdatasheet pdfdatasheet pdf datasheet pdf datasheet pdf400 Tests
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • The MitoPTTM JC-1 kit uses a quick and easy staining method to clearly differentiate between non-apoptotic and apoptotic cells through mitochondrial functionality.
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
      MitoPTTM JC-1 Reagent - lyophilized
      10X Assay Buffer, 125mL
      600uL vial of 50mM Carbonylcyanide m - chlorophenylhydrazone (CCCP)concentrate in DMSO
    • Preparing The Antibody
    • Test Principle
      An early indication of apoptosis involves a collapse in the electrochemical gradient across the mitochondrial membrane. Loss of mitochondrial membrane potential can be detected by a unique fluorescent cationic dye known as JC-1 (5,5’,6,6’-tetrachloro-1,1’,3,3’-tetraethylbenzamidazolocarbocyanin iodide) that has been incorporated into the MitoPTTM JC-1 kit.

      The MitoPTTM JC-1 reagent easily penetrates cells and enters the mitochondria. It aggregates in the mitochondria of non-apoptotic cells and fluoresces red, whilst in apoptotic cells it diffuses throughout the cell. Once dispersed, the reagent assumes a monomeric form and fluoresces green. This allows an easy distinction between non-apoptotic red fluorescent cells and apoptotic green fluorescent cells which can be read with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.
    • Buffer Solution
    • Storage
      Store the unopened kit (and each unopened component) at -20oC until the expiration date. Protect the MitoPTTM JC-1 reagent from light at all times.

      Once reconstituted, the 100X MitoPTTM JC-1 stock should be stored at -20oC protected from light.
    • Shelf Life
      Please see label for expiry date.
    • Acknowledgements
      MitoPTTM is a trademark of Immunochemistry Technologies, LLC.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT944.pdf

    Additional Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit Formats

    Formats Applications Sizes available
    Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit : JC-1 Dye (Dual Green/Red Fluorescence) F, IF 400 Tests
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Mitochondrial Permeability Transition:MitoPT™ JC-1 Kit thumbnail image 1

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