Methotrexate Antibody

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  • Sheep anti Methotrexate
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    6120-0004Edatasheet pdfdatasheet pdf0.5 ml
    6120-0004
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti methotrexate antibody recognizes Methotrexate, a drug originally manufactured as a chemotherapeutic agen for the treatment or a number of cancers. Methotrexate also acts as a disease-modifying anti-rheumatic drug used for the treatment of autoimmune conditions such as rheumatoid arthritis, Chron's disease and vasculitis.

      Sheep anti methotrexate antibody exhibits the following cross reactivities with related compounds:-

      Aminopterin18%
      Folic acid<0.01%
      Dyhydrofolic acid<0.01%
      Tetrahydrofolic acid<0.01%
      N-5-Methyltetrahydrofolic acid<0.01%
      Folinic acid<0.01%
      N-(p-Aminobenzoyl)-L-glutamic acid<0.01%
    • Intended Use
    • Target Species
      Chemical
    • Product Form
      Serum - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Methotrexate (4-amino-10-methylpteroyl-L-glutamic acid) conjugated via the carboxyl groups of the glutamic acid residue to KLH.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Methotrexate Antibody Formats

    Formats Applications Sizes available
    Methotrexate Antibody : Serum E 0.5 ml
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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