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Poly Caspase

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SR FLICA™ Poly Caspase Kit

Product Type
Kits
Specificity
Poly Caspase

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Product Code Applications Pack Size List Price Your Price Qty
ICT917
Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 100 Tests loader
List Price Your Price
loader
ICT916
Datasheet Datasheet
SDS Safety Datasheet SDS
F IF 25 Tests loader
List Price Your Price
loader
SR FLICA™ Poly Caspase Kit thumbnail image 1
SR FLICA™ Poly Caspase Kit gallery image 1
Staining of staurosporine-induced Jurkat cells using the SR FLICA poly caspase kit.

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IF

The SR FLICA Poly Caspase Kit uses a quick and easy method to analyze active caspases in apoptotic cells. This FLICA reagent binds the following activated caspases: caspase 1, caspase 3, caspase 4, caspase 5, caspase 6, caspase 7, caspase 8 and caspase 9. It can also be used as a generic probe for the detection of most caspases.

Reagents In The Kit
ICT917: 4 vials of SR-VAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Hoechst 33342, 1 mL.
ICT916: 1 vial of SR-VAD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst 33342, 1 mL.
Test Principle
ICT917
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.

The caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
ICT916
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.

The Caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Sulforhodamine 565 590
Regulatory
For research purposes only.
Guarantee
Guaranteed until date of expiry. Please see product label.
Acknowledgements
FLICA is a trademark of Immunochemistry Technologies, LLC.

This product is shipped at ambient temperature.
Store the unopened kit and each unopened component at +4°C until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20°C for 6 months protected from light and thawed no more than twice during that time.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Instructions For Use
ICT917
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT917.pdf
ICT916
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT916.pdf

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
FAM FLICA™ Caspase-3/7 Kit ICT094 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-3/7 Kit
FAM FLICA™ Caspase-6 Kit ICT096 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-6 Kit
FAM FLICA™ Caspase-1 Kit ICT098 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-1 Kit
FAM FLICA™ Caspase-8 Kit ICT910 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-8 Kit
FAM FLICA™ Caspase-9 Kit ICT913 F IF 100 Tests
List Price Your Price
Description FAM FLICA™ Caspase-9 Kit
FAM FLICA™ Caspase-3/7 Kit ICT093 F IF 25 Tests
List Price Your Price
Description FAM FLICA™ Caspase-3/7 Kit
FAM FLICA™ Caspase-6 Kit ICT095 F IF 25 Tests
List Price Your Price
Description FAM FLICA™ Caspase-6 Kit
FAM FLICA™ Caspase-1 Kit ICT097 F IF 25 Tests
List Price Your Price
Description FAM FLICA™ Caspase-1 Kit
FAM FLICA™ Caspase-8 Kit ICT099 F IF 25 Tests
List Price Your Price
Description FAM FLICA™ Caspase-8 Kit
FAM FLICA™ Caspase-9 Kit ICT912 F IF 25 Tests
List Price Your Price
Description FAM FLICA™ Caspase-9 Kit
SR FLICA™ Poly Caspase Kit ICT917 F IF 100 Tests
List Price Your Price
Description SR FLICA™ Poly Caspase Kit

References for Poly Caspase

  1. Soni, C. et al. (2015) Distinct and synergistic roles of FcγRIIB deficiency and 129 strain-derived SLAM family proteins in the development of spontaneous germinal centers and autoimmunity.
    J Autoimmun. 63: 31-46.
  2. Wong, E.B. et al. (2015) B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance.
    J Immunol. 194 (9): 4130-43.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"