Poly Caspase

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SR FLICA™ Poly Caspase Kit

Product Type
Kits
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
ICT916 F IF 25 Tests
ICT917 F IF 100 Tests
The SR FLICA™ Poly Caspase kit uses a quick and easy method to analyze active caspases in apoptotic cells.
This FLICA™ reagent binds the following activated caspases: Caspase 1, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8 and Caspase 9. It can also be used as a generic probe for the detection of most caspases.

Product Details

Reagents in the Kit
Pack Size: 25 Tests
1 vial of SR-VAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 15 mL
Fixative, 6 mL
Hoechst Stain, 1 mL
Pack Size: 100 Tests
4 vials of SR-VAD-FMK FLICA™ Reagent - lyophilized
10X Wash Buffer, 60 mL
Fixative, 6 mL
Hoechst Stain, 1 mL
Test Principle
Caspase FLICA™ kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA™ reagent contains a caspase inhibitor sequence linked to a red (Sulforhodamine, SR) fluorescent probe.

The Caspase FLICA™ Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage
Store the unopened kit (and each unopened component) at +4°C until the expiration date.
Protect the FLICA™ reagent from light at all times.

Once reconstituted, the 150X FLICA™ stock should be stored at -20°C protected from light.
Shelf Life
Please see label for expiry date.

More Information

Acknowledgements
FLICA™ is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Poly Caspase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT916.pdf

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
FAM FLICA™ Caspase-3/7 Kit ICT093 25 Tests F IF
FAM FLICA™ Caspase-6 Kit ICT095 25 Tests F IF
FAM FLICA™ Caspase-1 Kit ICT097 25 Tests F IF
FAM FLICA™ Caspase-8 Kit ICT099 25 Tests F IF
FAM FLICA™ Caspase-9 Kit ICT912 25 Tests F IF
SR FLICA™ Poly Caspase Kit ICT917 100 Tests F IF
FAM FLICA™ Caspase-2 Kit ICT918 25 Tests F IF
FAM FLICA™ Caspase-3/7 Kit ICT094 100 Tests F IF
FAM FLICA™ Caspase-6 Kit ICT096 100 Tests F IF
FAM FLICA™ Caspase-1 Kit ICT098 100 Tests F IF
FAM FLICA™ Caspase-8 Kit ICT910 100 Tests F IF
FAM FLICA™ Caspase-9 Kit ICT913 100 Tests F IF
FAM FLICA™ Caspase-10 Kit ICT923 100 Tests F IF

Product Specific References

References for Poly Caspase

  1. Soni, C. et al. (2015) Distinct and synergistic roles of FcγRIIB deficiency and 129 strain-derived SLAM family proteins in the development of spontaneous germinal centers and autoimmunity.
    J Autoimmun. 63: 31-46.
  2. Wong, E.B. et al. (2015) B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance.
    J Immunol. 194 (9): 4130-43.