Poly Caspase

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FLICA™ 660 Poly Caspase Kit

Product Type
Product Code Applications Pack Size List Price Quantity
25 - 50 Tests

The FLICA 660 Poly Caspase kit uses a quick and easy method to analyze active caspases in apoptotic cells.
This FLICA reagent binds the following activated caspases: Caspase 1, Caspase 3, Caspase 4, Caspase 5, Caspase 6, Caspase 7, Caspase 8 and Caspase 9. It can also be used as a generic probe for the detection of most caspases.

Product Details

Reagents in the Kit
1 vial of 660-VAD-FMK FLICA Reagent - lyophilized
10x Wash Buffer, 15 mL
Fixative, 6 mL
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a far-red fluorescent 660 dye.

The Caspase FLICA Kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits. This kit can be used with either a flow cytometer or fluorescence microscope.

Storage Information

Store the unopened kit (and each unopened component) at +4oC until the expiration date.
Protect the FLICA reagent from light at all times.

Once reconstituted in DMSO, the FLICA stock should be stored at -20°C protected from light.
Guaranteed until date of expiry. Please see product label.

More Information

FLICA is a trademark of Immunochemistry Technologies, LLC.
For research purposes only

Applications of Poly Caspase

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT9120.pdf

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
FLICA™ 660 Caspase-1 Kit ICT9122 F IF 25 - 50 Tests
FLICA™ 660 Caspase-3/7 Kit ICT9125 F IF 25 - 50 Tests