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Caspase-3/7

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FAM FLICA™ Caspase-3/7 Kit thumbnail image 1 FAM FLICA™ Caspase-3/7 Kit thumbnail image 2
FAM FLICA™ Caspase-3/7 Kit gallery image 1
Both cells are apoptotic (green) and dying (blue nuclei). As the left cell is much brighter green than the right cell, the left cell had more active caspases
FAM FLICA™ Caspase-3/7 Kit gallery image 2
Jurkat cells were treated as shown with staurosporine at 1mM to induce apoptosis. The cells were then stained with Caspase 3/7 FAM FLICA™ (ICT093) and ReadiDrop™™propidium iodide (1351101). Apoptotic cells positive for caspases can be seen in the bottom right quadrant and dead cells positive for both caspase and PI in the top right quadrant. Healthy cells are negative for both stains. Data acquired on the ZE5™ Cell Analyzer.

FAM FLICA™ Caspase-3/7 Kit

Product Type
Kits
Product Code Applications Pack Size List Price Quantity
ICT093
Datasheet
F IF 25 Tests

ICT094
Datasheet
F IF 100 Tests

The FAM FLICA™ Caspase 3 & 7 kit uses a quick and easy method to analyze active caspases in apoptotic cells.

Product Details

Reagents in the Kit
Pack Size: 25 Tests
1 vial of FAM-DEVD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 15 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL
Pack Size: 100 Tests
4 vials of FAM-DEVD-FMK FLICA Reagent - lyophilized
10x Apoptosis Wash Buffer, 60 mL
Fixative, 6 mL
Propidium Iodide, 1 mL
Hoechst 33342, 1 mL
Test Principle
Caspase FLICA kits measure apoptosis by detecting active caspases in whole, living cells. These kits do not work by using antibodies or as an ELISA. Instead, their methodology is based on a unique cell-permeable and non-cytotoxic reagent called the Fluorochrome Inhibitor of Caspases (FLICA). The FLICA reagent contains a caspase inhibitor sequence linked to a green (Carboxyfluorescein, FAM) fluorescent probe.

The caspase FLICA kits are suitable for cells in suspension and adherent cells from many species including mammalian, insect and yeast. Different cell types, e.g. HeLa, primary neurons, macrophages and lymphocytes have also been successfully studied with these kits.

This kit can be used with a flow cytometer, fluorescence microscope or a fluorescence plate reader using black microtitre plates.

Storage Information

Storage
Store the unopened kit and each unopened component at +4oC until the expiration date.
Once reconstituted with DMSO, use FLICA reagent immediately, or store at -20oC for 6 months protected from light and thawed no more than twice during that time.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

Acknowledgements
FLICA™ is a trademark of Immunochemistry Technologies, LLC.
Regulatory
For research purposes only

Applications of Caspase-3/7

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Instructions For Use
Instructions for use can be found at www.bio-rad-antibodies.com/uploads/IFU/ICT093.pdf
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
FAM FLICA™ Caspase-3/7 Kit ICT094 F IF 100 Tests

Product Specific References

References for Caspase-3/7

  1. Brugnon, F. et al. (2010) Apoptosis and meiotic segregation in ejaculated sperm from Robertsonian translocation carrier patients.
    Hum Reprod. 25 (7): 1631-42.
  2. Rébé, C. et al. (2007) Caspase-8 prevents sustained activation of NF-kappaB in monocytes undergoing macrophagic differentiation.
    Blood. 109 (4): 1442-50.
  3. Vacher, P. et al. (2015) Localized Store-Operated Calcium Influx Represses CD95-Dependent Apoptotic Effects of Rituximab in Non-Hodgkin B Lymphomas.
    J Immunol. 195 (5): 2207-15.

alamarBlue articles and technical help

Protocols

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Downloads

Instructions For Use ICT093
Instructions For Use ICT094
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