- Reagents In The Kit
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LNK012AP: 3 Vials of 100ug LYNX lyophilized AP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
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LNK014AP: 3 Vials of 10ug LYNX lyophilized AP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
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LNK011AP: 1 Vial of 100ug LYNX lyophilized AP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
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LNK013AP: 1 Vial of 1mg LYNX lyophilized AP mix
1 Vial LYNX Modifier reagent
1 Vial LYNX Quencher reagent
- Preparing The Antibody
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LNK012AP, LNK011AP: The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate*) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
The molar ratio of antibody: AP should be 1:1, i.e. 100ug antibody to every 100ug AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 100ul and a maximum antibody amount of 100ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.
* Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.
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LNK014AP: The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate*) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
The molar ratio of antibody: AP should be 1:1, i.e. 10ug antibody to every 10ug AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 10ul and a maximum antibody amount of 10ug. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.
* Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.
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LNK013AP: The following buffer solutions are recommended for preparing the antibody:
10-50mM amine-free buffer (e.g HEPES, MES, MOPS and phosphate*) pH range 6.5-8.5, although moderate concentrations of Tris buffer (<20mM) may be tolerated.
If possible, avoid buffers containing nucleophilic components such as primary amines and thiols (e.g. thiomersal/thimerosal) since they may react with LYNX chemicals. Azide (0.02-0.1%), EDTA and common non-buffering salts and sugars have little or no effect on conjugation efficiency.
The molar ratio of antibody: AP should be 1:1, i.e. 1mg antibody to every 1mg AP. For optimal results the antibody should be at a concentration of 1mg/ml, with a maximum volume of 1ml and a maximum antibody amount of 1mg. Antibody at a concentration of greater than 1mg/ml requires dilution. Antibody below 1mg/ml can still be used as long as the maximum volume is not exceeded. Using less than the recommended amount of antibody may result in unbound label, but this will be removed during subsequent application wash steps. Antibody below 0.5mg/ml should be concentrated before use with the kit.
* Phosphate may inhibit alkaline phosphatase activity. This kit can be used with antibodies prepared in phosphate buffer as long as the buffer will be washed away during an immunoassay procedure. Otherwise we recommend using a non-phosphate containing buffer for labeling antibodies with alkaline phosphatase.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch