Tubulin Beta 3 Antibody (PrecisionAbTM Antibody) | 5H2

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Tubulin Beta 3 Antibody (PrecisionAb<sup>TM</sup> Antibody) | 5H2 gallery image 1

Western blot analysis of whole cell lysates probed with tubulin beta 3 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11 second exposure. Arrow points to tubulin beta 3 (molecular weight 55 kDa)

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  • Mouse anti Tubulin Beta 3
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    Mouse anti human tubulin beta 3 antibody recognizes class III beta-tubulin, also known as TUBB4 or fibrosis of extraocular muscles, congenital, 3. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
    • Format
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00397WBdatasheet pdfdatasheet pdf100 µl
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      • Mouse anti Human tubulin Beta 3 antibody recognizes tubulin beta 3, also known as TUBB3, class III beta-tubulin and tubulin beta-III.

        The TUBB3 gene encodes a class III member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is primarily expressed in neurons and may be involved in neurogenesis and axon guidance and maintenance. Mutations in TUBB3 are the cause of congenital fibrosis of the extra-ocular muscles type 3. Alternate splicing results in multiple transcript variants. A pseudogene of TUBB3 is found on chromosome 6 (provided by RefSeq, Oct 2010).

        Mouse anti Human tubulin beta 3 antibody detects a band of 55 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
      • Target Species
      • Western Blotting
        Anti tubulin beta 3 detects a band of approximately 55 kDa in mouse and human brain tissue
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Recombinant protein fragment corresponding to aa 1-150 of human tubulin beta 3 (NP_006077) produced in E.coli
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
      • Lysate Reconstitution
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
      • Shelf Life
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        structural molecule activity
        microtubule-based movement
        GTP binding
        axon guidance
        protein polymerization
        'de novo' posttranslational protein folding
        GTPase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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