SIRT1 Antibody (PrecisionAbTM Antibody)

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SIRT1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with SIRT1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to SIRT1 (molecular weight 120 kDa)

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  • SIRT1 Antibody with Control Lysate
  • Rabbit anti SIRT1
  • SIRT1 Antibody with Control Lysate
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    Rabbit anti human SIRT1 antibody recognizes NAD-dependent protein deacetylase sirtuin-1, also known as SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1, sir2-like 1 or sirtuin type 1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00294WBdatasheet pdfdatasheet pdf100 µl
      VPA00294
      VPA00294KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00294K
      VPA00294KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00294KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
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      • Rabbit anti Human SIRT1 antibody recognizes SIRT1, also known as NAD-dependent deacetylase sirtuin-1, SIR2-like protein 1, SIR2alpha, hSIR2, hSIRT1, regulatory protein SIR2 homolog 1 and sirtuin type 1.

        The SIRT1 gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by SIRT1 is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants (provided by RefSeq, Dec 2008).

        Rabbit anti Human SIRT1 antibody detects a band of 120 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Rabbit IgG (H/L):HRPSTAR208P2 mlWB
        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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