PCNA Antibody

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PCNA Antibody gallery image 1

Staining of formalin fixed,paraffin embedded human breast with Rabbit anti Human PCNA (AHP1419)

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PCNA Antibody gallery image 2

Western blot analysis of PCNA expression in Jurkat (lane 1) and HEK293 (lane 2) whole cell lysates using Rabbit anti Human PCNA polyclonal antibody (AHP1419) followed by goat anti Rabbit IgG and visualized by immunofluorescence

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PCNA Antibody gallery image 3

Published customer image:
Rabit anti Human PCNA antibody (AHP1419) used for the identification of proliferating cells in the embryos of the Nile crocodile (Crocodylus niloticus), Bearded dragon (Pogona vitticeps) and Corn snake (Pantherophis guttatus) by immunofluorescence.
Image caption:
Development of epidermal scales during reptilian embryogenesis.
(A) Hematoxylin and eosin (H&E) staining of skin sections from different body regions (indicated with red arrows on the top insets with lateral views of corresponding embryos) of C. niloticus (crocodile; top row), P.vitticeps (lizard; two middle rows), and P. guttatus (snake; bottom row) embryos at various developmental stages [indicated as embryonic days (E) after oviposition]. White arrowheads indicate the anatomical placode. Scale bars, 100 μm. (B) Anatomical placodes in C. niloticus (left panels), P.vitticeps (middle panels), and P. guttatus (right panels) embryos. For each species, the whole-embryo WMISH with Sonic hedgehog (Shh) is shown (left panel) as well as, from top to bottom, high magnification of H&E-stained placode sections (white arrowheads indicate placode columnar cells), immunohistochemistry with PCNA (proliferation marker; epidermal-dermal junction indicated by dashed white lines), and parasagittal cryosections of placodes after Shh or β-catenin (Ctnnb1) WMISH. Bmp4 is also shown for lizard. Red double-headed arrows indicate the body region processed for sectioning.

From: Di-Poi, N & Milinkovitch, M.C. (2016)
The anatomical placode in reptile scale morphogenesis indicates shared ancestry among skin appendages in amniotes.
Sci Adv. 2: 1600708.

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  • Rabbit anti PCNA
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1419E, P *, WBdatasheet pdfdatasheet pdf50 µg
    AHP1419
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human PCNA antibody recognizes Human Proliferating Cell Nuclear Antigen (PCNA), also known as cyclin. PCNA is a 261 amino acid ~36kDa nuclear protein vital for cellular DNA synthesis, playing a key role in the DNA damage response where it acts as a loading platform to recruit additional damage repair proteins to lesions following DNA damage and promote repair (Burkovics et al. 2009).

      PCNA is a useful marker for DNA synthesis and is highly conserved among most species. Rabbit anti human PCNA antibody is expected to have wide species cross reactivity. PCNA expression in human dermis is negatively correlated to age as demonstrated by immunohustochemistry on paraffin sections using rabbit anti human PCNA antibody (Gunin et al. 2014).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/20001/8000
      Western Blotting1/5001/2000
      Immunohistology - Paraffin(1)2.5ug/ml
      (1)
      This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Breast
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional PCNA Antibody Formats

    Formats Applications Sizes available
    PCNA Antibody : Purified E, P *, WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          BUF025C
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P
          Histar Detection SystemSTAR3000B150 TestsC, P
          STAR3000B
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP
          BUF025A

          Recommended Positive Controls

            Histology Controls

              Breast
              • Application NameReference Images
                Immunohistology - Paraffin

              References

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