NFkB p65 Antibody

//www.bio-rad-antibodies.com/human-nfkb-p65-antibody-ahp288.html
NFkB p65 Antibody gallery image 1

Published customer image:
Rabbit anti NFκBp65 antibody used for the evaluation of NF&kappab expression in muring kidney by immunofluorescence.
Image caption:
Response of vascular endothelial cells in human kidney in organ culture to TL1A. Confocal images of kidney organ culture incubated with either culture media alone or with TL1A (0.2 µg/ml) or TNF (5 ng/ml) for 3 hours at 37°C. (A-C) cultures incubated in media alone show EC staining for NF-κBp65 (green) in both the glomerular (inset) and in peritubular vassels. Some peritubular vessels EC showed positive staining for DR3 (red). (D-F) Cultures treated with TL1A show co-staining for NF-κBp65 (green) and DR3 (red) in EC of some blood vessel (arrows). Inset; show no signal for NF-κBp65 on glomerular EC. (G-I) In contrast, TNF-treated cultures show a strong signal for NF-κB p65 (green) in EC of glomerular (inset) and peritubular blood vessels. DR3 (red) is present only in EC of peritubular blood vessels negative for NF-κB p65. (Original Mags; x40).

From: Wang J, Al-Lamki RS, Zhu X, Liu H, Pober JS, Bradley JR. TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.
BMC Nephrol. 2014 Nov 16;15:178.

Enlarge
NFkB p65 Antibody gallery image 2

Published customer image:
Rabbit anti NFκBp65 antibody used for the evaluation of NF&kappab expression in muring kidney by immunofluorescence.
Image caption:
TL1a induced NF-κB activation in renal interstitial vascular endothelial cells in mouse organ cultures. Kidney tissue from DR3 wild type (wt) and DR3 knockout (ko) mice were treated with TL1A or TNF and immunolabeled as described in materials and methods. (A-C) Untreated (UT) DR3wt showed no positive staining for NF-κB activation in CD31 positive EC from glomerulus or interstitial vessels. (D-F) some interstitial EC were positive for NF-κB activation after TL1A treatment. (G-I) more vascular EC were positive for NF-κB activation after TNF treatment. (J-L) untreated DR3ko showed no staining for NF-κB activation in vascular EC. (M-O) TL1A-treated DR3ko showed no staining for NF-κB activation in vascular EC. (P-R) TNF-treated DR3ko showed positive staining for NF-κB activation in vascular EC. Arrow for BvEC: blood vessel endothelial cells. Results were representative of 3 experiments.

From: Wang J, Al-Lamki RS, Zhu X, Liu H, Pober JS, Bradley JR. TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.
BMC Nephrol. 2014 Nov 16;15:178.

Enlarge
NFkB p65 Antibody gallery image 3

Published customer image:
Rabbit anti NFκBp65 antibody used for the evaluation of NF&kappab expression in muring kidney by immunofluorescence.
Image caption:
TL1A induced NF-κB activation in vascular endothelial cells in mouse heart organ cultures. (6a) Heart tissue from DR3 wild type (wt) and DR3 knock-out (ko) mice were treated with TL1A or TNF and immunolabeled as described in materials and methods. DR3wt showed more nuclear NF-κB activation in CD31 positive EC after TL1A treatment (B) compared to the untreated (A). There was similar odd nuclear NF-κB activation in DR3ko mice EC between TL1A treated (E) and untreated cultures (D). TNF induced a more profound nuclear NF-κB activation in EC of both DR3wt and DR3ko (C and F). Results were representative of 3 experiments. (6b) Graph showed a statistical difference in number of EC in TL1A treated tissue with NF-κB activation in DR3wt compared to DR3ko (p?<?0.05) (*).

From: Wang J, Al-Lamki RS, Zhu X, Liu H, Pober JS, Bradley JR. TL1-A can engage death receptor-3 and activate NF-kappa B in endothelial cells.
BMC Nephrol. 2014 Nov 16;15:178.

Enlarge
  • NFkB p65 Antibody thumbnail image 1
  • NFkB p65 Antibody thumbnail image 2
  • NFkB p65 Antibody thumbnail image 3
  • Rabbit anti NFkB p65
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP288E, GS, IF, IP, WBdatasheet pdfdatasheet pdf0.1 ml
    AHP288
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Rabbit anti Human NFkB p65 antibody recognizes the human Transcription factor p65, also known as Nuclear factor NF-kappa-B p65 subunit, NFκB p65, RelA or Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3. NFκB p65 is a 551 amino acid ~65 kDa subunit of the NFκB transcription factor present in most cell types and is involved as the endpoint in an array of signal transduction events related to many processes including apotosis, immunity, inflammation and tumorigenesis. The most abundant form of NFκB appears to be the p65-p50 heterodimer. In the active form NFκB p65 is found in the nucleus co-localizing with the ATP-dependent RNA helicase DDX1. NFκB p65 is also found in an inactive form in the cytoplasm associated with the inhibitor IκB (UniProt: Q04206)

      In a gel supershift assay Rabbit anti Human NFκB p65 antibody was found to be active against all p65 containing human, mouse or rat NFκB complexes using 0.5ul to 1.0ul per assay. The binding of Rabbit anti Human NFκB p65 antibody to the NFκB p65 subunit can be blocked using the NFκB p65 control peptide (PHP073).
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/25000
      Gel Super Shift Assays0.51.0 ul per test
      Immunofluorescence
      Immunoprecipitation
      Western Blotting1/20001/5000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional NFkB p65 Antibody Formats

    Formats Applications Sizes available
    NFkB p65 Antibody : Serum E, GS, IF, IP, WB 0.1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      STAR121B
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      2AB02B
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF
      STAR36D488GA
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      STAR36D549GA
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      STAR36D649GA
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      STAR36D800GA
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      STAR121F
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      STAR34B
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      STAR121P
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      STAR124P
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      STAR54
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF
      STAR35A

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            NFkB p65 Control PeptidePHP07350 µgFN
            PHP073

            Histology Controls

              • Application NameReference Images
                Western Blotting

              References

              Write your review

              You may also be interested in...

              You May Also Be Interested In...