IL-4 antibody | MP4-25D2

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Rat anti Human Interleukin-4:Alexa Fluor® 488

Product Type: Monoclonal Antibody
Clone: MP4-25D2
Isotype: IgG1
Specificity: IL-4

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References (4)
Applications
Storage Information
Batch/Lot Specific Datasheets

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MCA1525A488 Datasheet SDS SDS	F *	100 Tests/1ml		Log in
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Anti Human Interleukin-4 Antibody, clone MP4-25D2 thumbnail image 1

Anti Human Interleukin-4 Antibody, clone MP4-25D2 gallery image 1 — Western blot analysis of human IL-4 recombinant protein probed with Rat anti Human Interleukin-4 (**MCA1525**) followed by F(ab')₂ Rabbit anti Rat IgG:HRP (STAR21B).

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Rat anti Human Interleukin-4 antibody, clone MP4-25D2 recognizes human Interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, Binetrakin, Pitrakinra or Lymphocyte stimulatory factor 1. Interleukin-4 is a 153 amino acid ~17kDa cytokine involved in the activation of B cells, macrophages and monocytes (UniProt: P05112).

Polymorphisms in the IL-4 gene are associated with susceptibility to, and outcome of ischemic stroke (Marousi et al. 2011).

Rat anti Human Interleukin-4 antibody, clone MP4-25D2 is reported to neutralize IL-4 activity (Abrams et al. 1992 and Carvalho et al. 1994).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Rhesus Monkey; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG conjugated to Alexa Fluor® 488 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Purified CHO cell expressed recombinant human IL-4.
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Alexa Fluor®488 495 519
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

Target Species	Cross Reactivity
Rhesus Monkey

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®488	495	519

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		NT	1/10

¹ Membrane permeabilization is required for this application. The use of Leucoperm (Product Code BUF09 ) is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to label 1x10⁶ cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸

Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra

References for IL-4 antibody

Abrams, J.S. et al. (1992) Strategies of anti-cytokine monoclonal antibody development: immunoassay of IL-10 and IL-5 in clinical samples.
Immunol Rev. 127: 5-24.
Carvalho, E.M. et al. (1994) Restoration of IFN-gamma production and lymphocyte proliferation in visceral leishmaniasis.
J Immunol. 152 (12): 5949-56.
P, M. et al. (2004) Tumor-derived MUC1 mucins interact with differentiating monocytes and induce IL-10highIL-12low regulatory dendritic cell.
J Immunol. 172 (12): 7341-9.
Raulf-Heimsoth, M. (2008) T cell - primary culture from peripheral blood.
Methods Mol Med. 138: 17-30.

UniProt: P05112
Entrez Gene: IL4
GO Terms: GO:0005125 cytokine activity; GO:0005136 interleukin-4 receptor binding; GO:0005615 extracellular space; GO:0006935 chemotaxis; GO:0006968 cellular defense response; GO:0008083 growth factor activity; GO:0008203 cholesterol metabolic process; GO:0016563 transcription activator activity; GO:0016564 transcription repressor activity; View More GO Terms; GO:0030183 B cell differentiation; GO:0030890 positive regulation of B cell proliferation; GO:0042092 type 2 immune response; GO:0043066 negative regulation of apoptosis; GO:0045189 connective tissue growth factor biosynthetic process; GO:0045348 positive regulation of MHC class II biosynthetic process; GO:0045671 negative regulation of osteoclast differentiation; GO:0045944 positive regulation of transcription from RNA polymerase II promoter; GO:0048295 positive regulation of isotype switching to IgE isotypes; GO:0048304 positive regulation of isotype switching to IgG isotypes