IL-16 Antibody

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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP454GE, FN, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP454G
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human Interleukin-16 antibody recognizes human IL-16 (Interleukin-16), originally known as leucocyte chemoattractant factor (LCF), an immunomodulatory cytokine which induces the chemotaxis of CD4 expressing T cells, eosinophils and monocytes and is produced constitutively by T cells, eosinophils, dendritic cells, fibroblasts and neuronal cells.

      The biologically active form of IL-16 is generated by enzymatic cleavage of the IL-16 precursor (pro-IL16), producing a peptide corresponding to the 121 amino acid C-Terminal portion. Further properties attributed to IL-16 include the induction of MHC class II and IL-2 receptor (IL-2R) expression, and several studies have indicated a role for IL-16 in inflammatory processes.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - lyophilised
    • Reconstitution
      Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
    • Preparation
    • Antiserum Preparation
      Antisera to human IL-16 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
    • Preservative Stabilisers
      None present
    • Immunogen
      Recombinant human IL-16
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at -20oC.
      This product should be stored undiluted.
      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0.09% Sodium Azide is recommended.
    • Shelf Life
      12 months from date of reconstitution.
    • GO Terms
      cytokine activity
      transcription
      plasma membrane
      immune response
      extracellular space
      interspecies interaction between organisms
      regulation of transcription
      cytoplasm
      nucleus
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA0.5 ug/ml
      Functional Assays1270ng/ml
      Western Blotting0.10.2ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use
      For functional studies do not add Azide.

    Additional IL-16 Antibody Formats

    Formats Applications Sizes available
    IL-16 Antibody : Purified E, FN, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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