Rabbit anti Human Interleukin-16 antibody recognizes human IL-16 (Interleukin-16), originally known as leucocyte chemoattractant factor (LCF), an immunomodulatory cytokine which induces the chemotaxis of CD4 expressing T cells, eosinophils and monocytes and is produced constitutively by T cells, eosinophils, dendritic cells, fibroblasts and neuronal cells.
The biologically active form of IL-16 is generated by enzymatic cleavage of the IL-16 precursor (pro-IL16), producing a peptide corresponding to the 121 amino acid C-Terminal portion. Further properties attributed to IL-16 include the induction of MHC class II and IL-2 receptor (IL-2R) expression, and several studies have indicated a role for IL-16 in inflammatory processes.
Purified IgG - lyophilised
Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Antisera to human IL-16 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by antigen affinity chromatography.
Recombinant human IL-16
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Reagents In The Kit
Preparing The Antibody
Phosphate buffered saline
Prior to reconstitution store at +4oC. Following reconstitution store at -20oC. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. For long term storage the addition of 0.09% Sodium Azide is recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.