IgM
Native Human IgM
- Product Type
- Antigen
- Specificity
- IgM
Antibody Quality and Performance Guarantee
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E Reset| Native Human IgM is purified from human plasma and demonstrated a single band in electrophoresis. Native Human IgM has been used as the IgM antigen in the development of a sensitive ELISA assay for the measurement of IgM in human samples. |
- Target Species
- Human
- Product Form
- Purified immunoglobulin from human plasma - lyophilized
- Reconstitution
- Reconstitute with 1ml 250mM Sodium Chloride. Further dilutions may then be made in sterile Phosphate Buffered Saline.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgM prepared by delipidation, salt fractionation and ion-exchange chromatography.
- Buffer Solution
- Phosphate buffered saline.
- Preservative Stabilisers
- None present.
- Purity
- >95% (Single band in high resolution protein electrophoresis on Sebia agarose gel).
IgM and kappa light chain confirmed via Sebia agarose immunofixation.
This product may contain traces of other plasma proteins, including IgGs. - Approx. Protein Concentrations
- 5.0 mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- RRID
- AB_872062
- UniProt
- P01871
- Entrez Gene
- IGHM
- GO Terms
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0005624 membrane fraction
View more products with IgM specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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