Human IgG2 | AbD264_hIgG2

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Human IgG2 | AbD264_hIgG2 gallery image 1

SDS-PAGE analysis of recombinant Human IgG2 lambda

Recombinant human IgG2 lambda was analyzed under non-reducing, denaturing conditions (Lane 2) and reducing, denaturing conditions (Lane 3). Bands are visualized using Coomassie staining and the heavy (HC) and light chains (LC) are indicated, respectively. The molecular weights (kDa) of the marker proteins (Lane 1) are indicated on the left hand side of the image

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Human IgG2 | AbD264_hIgG2 gallery image 2

Size exclusion chromatography of recombinant Human IgG2 lambda

UV280 chromatogram of a size exclusion chromatography run of recombinant Human IgG2 lambda. The larger peak corresponds to monomeric, fully assembled AbD264_hIgG2. The smaller shoulder most likely contains dimers and aggregates

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  • Human IgG2 | AbD264_hIgG2 thumbnail image 1
  • Human IgG2 | AbD264_hIgG2 thumbnail image 2
  • Recombinant Human IgG2 Lambda
  • Recombinant Human IgG2 Lambda
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Recombinant Protein
  • Clone
    AbD264_hIgG2
  • Isotype
    IgG2 Lambda
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    HCA108Edatasheet pdfdatasheet pdf0.1 mg
    HCA108
    HCA108AEdatasheet pdfdatasheet pdf50 µg
    HCA108A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Recombinant Human IgG2 lambda contains a lambda light chain. The heavy chain belongs to the G2m(..), previously designated G2m(n-), allotype.
      Recombinant Human IgG2 lambda is a recombinant antibody with specificity for Green Fluorescent Protein. It has no known reactivity with mammalian proteins or other antigens. It is therefore recommended as a control reagent for assays where this immunoglobulin is measured, or when using other human antibodies of the same isotype and subclass. It is recommended that this reagent is used at the same concentration as the test reagent.
    • Intended Use
    • Product Form
      Pack Size: 0.1 mgHuman IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
      Pack Size: 50 µgHuman IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line, HKB-11. This antibody is supplied lyophilised.
      Pack Size: 0.1 mgHuman IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line, HKB-11. This antibody is supplied liquid.
      Pack Size: 50 µgHuman IgG2 antibody selected from the HuCAL® phage display library and expressed in a human cell line, HKB-11. This antibody is supplied lyophilised.
    • Reconstitution
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
      Pack Size: 50 µgReconstitute with 0.5ml distilled water
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein A
      Purified IgG prepared by affinity chromatography on Protein A
    • Preservative Stabilisers
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
      Pack Size: 0.1 mg0.01% Thiomersal
      Pack Size: 50 µg0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin
    • Immunogen
      Green fluorescent protein
    • Purity
    • Approx. Protein Concentrations
      Pack Size: 0.1 mgTotal protein concentration 0.5 mg/ml
      Pack Size: 50 µgTotal protein concentration 0.1mg/ml
      Pack Size: 0.1 mgTotal protein concentration 0.5 mg/ml
      Pack Size: 50 µgTotal protein concentration 0.1mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 0.1 mgStore at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Pack Size: 50 µgPrior to reconstitution store at +4oC.
      After reconstitution store at -20oC.
      Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution.
      12 months from date of despatch.
    • GO Terms
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
      Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
    • Licensed Use
      For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Technical Advice
      Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional Human IgG2 Formats

    Formats Clone Applications Sizes available
    Human IgG2 : Purified AbD264_hIgG2 E 0.1 mg | 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Goat anti Human IgG F(ab')2:Alk. PhosSTAR126A0.5 mlE, WB
      STAR126A
      Goat anti Human IgG F(ab')2:BiotinSTAR126B0.5 mgC, E, F, WB
      STAR126B
      Goat anti Human IgG F(ab')2:FITCSTAR126F0.5 mgC, F, IF
      STAR126F
      Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
      MCA647P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
      STAR126P
      Goat anti Human IgG F(ab')2STAR1260.5 mgE
      STAR126
      Goat anti Human IgG F(ab')2:TritcSTAR126TRC0.5 mgC, F, IF
      STAR126TRC
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Human IgG F(ab')2:Alk. Phos.0500-01000.5 mlE, WB
      0500-0100
      Goat anti Human IgG F(ab')2:Alk. PhosSTAR126A0.5 mlE, WB
      STAR126A
      Goat anti Human IgG F(ab')2:BiotinSTAR126B0.5 mgC, E, F, WB
      STAR126B
      Goat anti Human IgG F(ab')2:FITCSTAR126F0.5 mgC, F, IF
      STAR126F
      Mouse anti Human IgG (Fc) CH2 Domain:HRPMCA647P0.2 mgC *, E
      MCA647P
      Goat anti Human IgG F(ab')2:HRP0500-00990.5 mlE, WB
      0500-0099
      Goat anti Human IgG F(ab')2:HRPSTAR126P0.5 mlE, WB
      STAR126P
      Goat anti Human IgG F(ab')2STAR1260.5 mgE
      STAR126
      Goat anti Human IgG F(ab')2:TritcSTAR126TRC0.5 mgC, F, IF
      STAR126TRC

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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