IgG Antibody

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Inhibition of Adalimumab binding to TNFα by HCA202. TNFα was used as the coating antigen followed by a pre-incubated mixture of Human anti Adalimumab (HCA202) titrated into a constant amount of Adalimumab. Free Adalimumab still capable of binding to the plate was detected using Goat anti-human Fab (STAR126P)

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  • IgG Antibody thumbnail image 1
  • Goat anti Human IgG F(ab')2
  • Goat anti Human IgG F(ab')2:HRP
  • Goat anti Human IgG F(ab')2:FITC
  • Goat anti Human IgG F(ab')2:Biotin
  • Goat anti Human IgG F(ab')2:Tritc
  • Goat anti Human IgG F(ab')2:Alk. Phos
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
6 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    STAR126Edatasheet pdfdatasheet pdf0.5 mg
    STAR126
    STAR126FC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    STAR126F
    STAR126BC, E, F, WBdatasheet pdfdatasheet pdf0.5 mg
    STAR126B
    STAR126TRCC, F, IFdatasheet pdfdatasheet pdf0.5 mg
    STAR126TRC
    STAR126PE, WBdatasheet pdfdatasheet pdf0.5 ml
    STAR126P
    STAR126AE, WBdatasheet pdfdatasheet pdf0.5 ml
    STAR126A
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Goat anti Human IgG F(ab')2 antibody recognizes Fab and F(ab')2 portion of human IgG by IEP. It reacts with other human immunoglobulins through common light chain reactivity.

      Goat anti Human IgG F(ab')2 antibody demonstrates minimal cross reactivity with bovine, horse and mouse IgG; other species have not been tested.
      No reactivity is seen with the Fc portion of human IgG or non-immunoglobulin serum proteins.

      This antibody is a performance tested, matched reagent for use with Bio-Rad HuCAL antibodies.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
      Purified IgG - liquid
      Purified Ig conjugated to Alkaline Phosphatase - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography.
      Purified IgG prepared by affinity chromatography
      Purified IgG prepared by affinity chromatography.
      Purified IgG prepared by affinity chromatography.
      Purified IgG prepared by affinity chromatography.
      Purified Ig prepared by affinity chromatography
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.01% Thiomersal
      1% Bovine Serum Albumin
      0.09% Sodium Azide (NaN3)
      1% Bovine Serum Albumin.
      0.09% Sodium Azide (NaN3)
      1.5% Bovine Serum Albumin.
      0.05% Sodium Azide (NaN3)
      1.5% Bovine Serum Albumin.
      0.05% Sodium Azide (NaN3)
      1.5% Bovine Serum Albumin
    • Immunogen
      Human IgG F(ab')2
    • Purity
    • Approx. Protein Concentrations
      1.0 mg/ml
      0.5 mg/ml
      1.0 mg/ml
      1.0 mg/ml
      1.0 mg/ml
      IgG concentration 0.6mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      Phosphate buffered saline
      TRIS buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      innate immune response
      complement activation, classical pathway
      protein binding
      extracellular region
      antigen binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Western Blotting1/10001/10000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
      Immunohistology - Frozen1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1,0001/10,000
      Flow Cytometry1/2001/1000
      Immunohistology - Frozen1/2001/1000
      Western Blotting1/1,0001/10,000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry1/501/200
      Immunofluorescence1/501/200
      Immunohistology - Frozen1/501/200
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10001/10000
      Western Blotting1/10001/10000

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Biotin C, E, F, WB 0.5 mg
    IgG Antibody : FITC C, F, IF 0.5 mg
    IgG Antibody : Purified E 0.5 mg
    IgG Antibody : HRP E, WB 0.5 ml
    IgG Antibody : Alk. Phos. E, WB 0.5 ml
    IgG Antibody : Tritc C, F, IF 0.5 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Goat IgG (Fc):FITCSTAR122F1 mgF
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      STAR122P
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              • Application NameReference Images
                Flow Cytometry

              References

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