This product, a Goat anti Human IgG (Fc) antibody conjugated to Alkaline Phosphatase, is specific for human IgG (Fc).Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, human IgG, human IgG (Fc) and human serum. No reaction was observed against human IgG F(ab')2.
Purified IgG conjugated to Alkaline Phosphatase - liquid
Purified IgG prepared from monospecific antiserum by immunoaffinity chromatography
0.1% Sodium Azide (NaN3) 50% Glycerol 1% Bovine Serum Albumin
Human IgG Fc fragment
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
TRIS buffered saline 0.001M Magnesium Chloride 0.0001M Zinc Chloride
Store at +4oC. DO NOT FREEZE. This product should be stored undiluted.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
1. Zeman, D. et al. (2012) Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite™ antibodies. comparison with the free light chain oligoclonal pattern.Fluids Barriers CNS. 9: 5.