hnRNP H1 Antibody (PrecisionAbTM Antibody)

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hnRNP H1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with hnRNP H1 antibody followed by detection with HRP conjugated Goat anti Rabbit (1/10,000, STAR208P) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to hnRNP H1 (molecular weight 50 kDa)

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  • hnRNP H1 Antibody with Control Lysate
  • hnRNP H1 Antibody with Control Lysate
  • Rabbit anti hnRNP H1
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    Rabbit anti human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00311WBdatasheet pdfdatasheet pdf100 µl
      VPA00311
      VPA00311KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00311K
      VPA00311KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00311KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Rabbit anti Human hnRNP H1 antibody recognizes heterogeneous nuclear ribonucleoprotein H1 (hnRNP H1).

        Encoded by the HNRNPH1 gene, hnRNP H1 is a member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hn RNPs). The hn RNPs are RNA binding proteins that complex with heterogeneous nuclear RNA. These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some may shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by the hnRNP H1 gene has three repeats of quasi-RRM domains that bind to RNA and is very similar to the family member HNRPF. HNRNPH1 may be associated with hereditary lymphedema type I. Alternatively spliced transcript variants have been described (provided by RefSeq, Mar 2012).

        Rabbit anti Human hnRNP H1 antibody detects a band of 50 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti hnRNP H1 detects a band of approximately 50 kDa in Raji cell lysate
        Anti hnRNP H1 detects a band of approximately 50 kDa in Raji cell lysate
        Anti hnRNP H1 detects a band of approximately 50 kDa in Raji cell lysate
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Rabbit polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Rabbit polyclonal antibody purified by affinity chromatography
        Rabbit polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        KLH-conjugated synthetic peptide corresponding to aa 48-77 of human hnRNP H1
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Raji lysate lyophilized in RIPA buffer
        400μg Raji lysate lyophilized in RIPA buffer
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles
      • Shelf Life
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
        As supplied, 12 months from date of despatch
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
        PrecisionAb™ is a trademark of Bio-Rad Laboratories
      • Regulatory
        For research purposes only
      • GO Terms
        cytoplasm
        poly(U) RNA binding
        nucleotide binding
        spliceosomal complex
        regulation of RNA splicing
        nucleoplasm
        nuclear mRNA splicing, via spliceosome
        protein binding
        actin cytoskeleton
        heterogeneous nuclear ribonucleoprotein complex
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

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        STAR208P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
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        STAR208P
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        STAR208P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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