GSK3 Alpha/Beta Antibody (PrecisionAbTM Antibody)

//www.bio-rad-antibodies.com/human-gsk3-alpha-beta-antibody-vma00342.html?RenderPageSpecific=1
GSK3 Alpha/Beta Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with GSK3 alpha/beta antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 11.1 second exposure. Arrow points to GSK3 alpha/beta (molecular weight 47/51 kDa)

Enlarge
  • GSK3 Alpha/Beta Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Mouse anti GSK3 Alpha/Beta
  • GSK3 Alpha/Beta Antibody with Control Lysate
  • GSK3 Alpha/Beta Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    GSK3 alpha /beta antibody recognizes glycogen synthase kinase-3 alpha also known as GSK-3 alpha or serine/threonine-protein kinase GSK3A and glycogen synthase kinase-3 beta, also known as GSK-3 beta or serine/threonine-protein kinase GSK3B. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2b
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00342WBdatasheet pdfdatasheet pdf100 µl
      VMA00342
      VMA00342KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00342K
      VMA00342KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00342KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human GSK3 alpha/beta antibody recognizes glycogen synthase kinase-3 (GSK3) alpha also known as glycogen synthase kinase-3 alpha, GSK-3 alpha and serine/threonine-protein kinase GSK3A and the glycogen synthase kinase-3 (GSK3) beta, also known as GSK-3 beta, GSK3beta isoform, glycogen synthase kinase-3 beta and serine/threonine-protein kinase GSK3B.

        The proteins encoded by GSK3A and GSK3B genes are a serine-threonine kinases, belonging to the glycogen synthase kinase subfamily. GSK3A is involved in Alzheimer and diabetes and GSK3B have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of GSK3B may be relevant to the pathogenesis of Alzheimer disease (provided by RefSeq, Sep 2009).

        Mouse anti Human GSK3 alpha/beta antibody detects bands of 51 and 47 kDa respectively. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti GSK3 alpha/beta detect bands of approximately 47 kDa and 51 kDa in HEK293 cell lysates
        Anti GSK3 alpha/beta detect bands of approximately 47kDa and 51 kDa in HEK293 cell lysates
        Anti GSK3 alpha/beta detect bands of approximately 47 kDa and 51 kDa in HEK293 cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        Ratyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
        0.09% Sodium Azide (NaN3)
      • Immunogen
        Synthetic peptide corresponding to amino acids 205-221 of human GSK3 beta
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg HEK293 lysate lyophilized in RIPA buffer.
        400μg HEK293 lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        beta-catenin binding
        axon guidance
        positive regulation of cell-matrix adhesion
        RNA polymerase II transcription factor binding
        negative regulation of glucose import
        protein phosphorylation
        cytosol
        negative regulation of protein complex assembly
        negative regulation of apoptosis
        epithelial to mesenchymal transition
        beta-catenin destruction complex
        NF-kappaB binding
        positive regulation of protein catabolic process
        nerve growth factor receptor signaling pathway
        hippocampus development
        phosphatidylinositol-mediated signaling
        positive regulation of protein export from nucleus
        ubiquitin protein ligase binding
        protein kinase A catalytic subunit binding
        tau-protein kinase activity
        canonical Wnt receptor signaling pathway
        negative regulation of protein binding
        superior temporal gyrus development
        positive regulation of Rac GTPase activity
        glycogen metabolic process
        ATP binding
        protein serine/threonine kinase activity
        p53 binding
        positive regulation of protein complex assembly
        peptidyl-serine phosphorylation
        negative regulation of insulin receptor signaling pathway
        negative regulation of transferase activity
        ER overload response
        Axin-APC-beta-catenin-GSK3B complex
        insulin receptor signaling pathway
        negative regulation of UDP-glucose catabolic process
        nucleus
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                New Mitochondrial Dynamics Poster

                Learn about the mitochondrial fusion-division cycle and how aberrant regulation results in disease

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted