GALECTIN-9 Antibody (PrecisionAbTM Antibody) | OTI1G3

//www.bio-rad-antibodies.com/human-galectin-9-antibody-oti1g3-vma00212.html?RenderPageSpecific=1
GALECTIN-9 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1G3 gallery image 1

Western blot analysis of whole cell lysates probed with galectin-9 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 21 second exposure. Arrow points to galectin-9 (molecular weight 40 kDa)

Enlarge
  • GALECTIN-9 Antibody (PrecisionAb<sup>TM</sup> Antibody) | OTI1G3 thumbnail image 1
  • Mouse anti GALECTIN-9
  • GALECTIN-9 Antibody with Control Lysate
  • GALECTIN-9 Antibody with Control Lysate
(Rated 0.0 out of 5 based on 0 customer reviews)
    Galectin-9 antibody recognizes Galectin-9, also known as ecalectin, Gal-9, tumor antigen HOM-HD-21 or urate transporter/channel protein. This antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Monoclonal
    • Isotype
      IgG2a
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VMA00212WBdatasheet pdfdatasheet pdf100 µl
      VMA00212
      VMA00212KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VMA00212K
      VMA00212KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VMA00212KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Mouse anti Human galectin-9 antibody recognizes galectin-9, also known as ecalectin, gal-9, galectin 9, tumor antigen HOM-HD-21 and urate transporter/channel protein.

        The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The protein encoded by the LGALS9 gene is an S-type lectin. It is overexpressed in Hodgkin's disease tissue and might participate in the interaction between the H&RS cells with their surrounding cells and might thus play a role in the pathogenesis of this disease and/or its associated immunodeficiency. Multiple alternatively spliced transcript variants have been found for LGALS9 (provided by RefSeq, Jul 2008).

        Mouse anti Human galectin-9 antibody detects a band of 40 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. This antibody is available with a positive control lysate, in a smaller trial size, and as a standalone antibody.
      • Target Species
        Human
      • Western Blotting
        Anti galectin-9 detects a band of approximately 40 kDa in Jurkat cell lysates
        Anti galectin-9 detects a band of approximately 40 kDa in Jurkat cell lysates
        Anti galectin-9 detects a band of approximately 40 kDa in Jurkat cell lysates
      • Species Cross-Reactivity
        Target SpeciesCross Reactivity
        Mouseyes
        N.B. Antibody reactivity and working conditions may vary between species.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 10 Westerns100μl Mouse monoclonal antibody purified by affinity chromatography from ascites
        Pack Size: 2 Westerns20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
      • Buffer Solution
        Phosphate buffered saline
        Phosphate buffered saline
        Phosphate buffered saline
      • Preservative Stabilisers
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
        0.09% Sodium Azide (NaN3)
        1% Bovine Serum Albumin
        50% Glycerol
      • Immunogen
        Full length human recombinant human galectin-9 (NP_002299) produced in HEK293T cells
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        cytoplasm
        positive regulation of I-kappaB kinase/NF-kappaB cascade
        signal transducer activity
        extracellular region
        galactose binding
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Goat anti Mouse IgG (H/L):HRPSTAR207P2 mlWB
        STAR207P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

                Write your review

                Western Blotting without Trial and Error: Use PrecisionAb™ Antibodies

                Watch how PrecisionAb Validated Western Blotting Antibodies are defining a new standard of quality that you can trust to deliver superior results and reproducibility in western blotting.

                Contact Information


                Online Payment Methods:

                1. Invoice / Purchase Order
                2. Credit Card (Maestro, VISA,MasterCard & VISA Electron)
                credit cards accepted