DISC1 Antibody

DISC1 Antibody gallery image 1

Western blot analysis of whole cell lysate from SK-N-SH human neuroblastoma cells probed with Rabbit anti Human DISC1 (AHP1446) at 0.5(A), 1(B) and 2(C) ug/ml

DISC1 Antibody gallery image 2

Immunocytochemical staining of HeLa cells with Rabbit anti Human DISC1 (AHP1446)

DISC1 Antibody gallery image 3

Published customer image:
Rabbit anti DISC1 antibody used forthe detection of DISC1 in mouse photoreceptors by immunofluorescence.
Image caption:
Colocalization of various Pericentrin interaction partners at the basal body complex of mouse photoreceptors. (A) Scheme of a vertebrate rod photoreceptor. (B–F) Micrographs of a vertical cryostat section through an adult mouse retina double labeled for CG-NAP (B, green) and Cen3 (C, red) as a marker for the ciliary apparatus (the connecting cilium (CC) and the basal body complex (BBC)) and the centrosomes. (B) CG-NAP and (C) Cen3 are localized in the ciliary region of the photoreceptors and at the centrosomes of the other retinal cells. (D) The merge of the stainings with the additional DAPI nuclear staining (blue) shows the partial colocalization of CG-NAP and Cen3 in the ciliary region of the photoreceptors and the centrosomes of the other retinal cells. (E–F) High power views showing the localization of CG-NAP at the BBC of the connecting cilium (E) and at the centrosomes of the other retinal cells (F). (G) Scheme of the ciliary apparatus. (H–K) High power views showing the localization of the Pcnt interaction partners PCM1 (H, green), DISC1 (I, green), IFT20 (J, green), and PC2 (K, green) at the BBC of the connecting cilium of the photoreceptor. Cen3 (red) marks the whole cilium with the ciliary apparatus. RPE: retinal pigment epithel; OS: outer segment; IS: inner segment; ONL: outer nuclear layer; OPL: outer plexiform layer; BB: basal body; CC: connecting cilium; C: centriole; CP: calycal process; MTs: microtubules; N: nucleus; S: synapse. Scale bars: 10 µm (B), 1 µm (E, F, J, K, H, I).

From: Mühlhans J, Brandstätter JH, Gießl A (2011) The Centrosomal Protein Pericentrin Identified at the Basal Body Complex of the Connecting Cilium in Mouse Photoreceptors.
PLoS ONE 6(10): e26496. doi:10.1371/journal.pone.0026496

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  • Rabbit anti DISC1
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1446C, WBdatasheet pdfdatasheet pdf0.1 mg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
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    • Rabbit anti DISC1 antibody recognizes disrupted in schizophrenia 1 (DISC1), ~93 kDa protein located in the nucleus, cytoplasm and mitochondria. The DISC1 protein is widely expressed in normal tissues. It is involved in neurite outgrowth and cortical development through its interaction with a variety of proteins including lissencephaly-1 (LIS1), NudeE-like (NUDEL) and phosphodiesterase 4B.

      In certain cases of schizophrenia the DISC1 gene has been found to be disrupted by a t(1;11) (q42.1;q14.3) translocation, and studies suggest that DISC1 could be a candidate susceptibility gene for this condition.

      Rabbit anti DISC1 antibody has been found suitable for immunocytochemistry (ICC) at a concentration of 5ug/ml.
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Antisera to human DISC1 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      An 18 amino acid peptide located near the centre of human DISC1.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting12ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      AHP1446 detects bands of approximately 95-100kDa in SK-N-SH cell lysate.
    • Instructions For Use

    Additional DISC1 Antibody Formats

    Formats Applications Sizes available
    DISC1 Antibody : Purified C, WB 0.1 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*

          Recommended Positive Controls

            Histology Controls


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