Delta-Like Protein 4 Antibody

Delta-Like Protein 4 Antibody gallery image 1

Immunohistochemical staining of formalin fixed, paraffin embedded human ovary with Rabbit anti Human DLL4 antibody (AHP1274)

Delta-Like Protein 4 Antibody gallery image 2

Western blot analysis of Delta-like Protein 4 expression in murine pancreatic lysate using Rabbit anti Human Detla-like Protein 4 antibody followed by HRP conjugated Goat anti Rabbit IgG antibody and visualized by chemiluminescence

Delta-Like Protein 4 Antibody gallery image 3

Published customer image:
Rabbit anti Human Dll4 antibody (AHP1274) used for the identification of Dll4 expressing cells in atherosclerotic plaques by immunohistochemistry.
Image caption:
Example of immunostaining of different antibodies.
A: Overview. C: CD68 positive macrophages. E: VEGFR2. F: VEGF. H: Dll4. Black bars represent 1000 μm (A) and 200 μm (B-H).

From: Hjelmgren O, Gellerman K, Kjelldahl J, Lindahl P, Bergström GML (2016)
Increased Vascularization in the Vulnerable Upstream Regions of Both Early and Advanced Human Carotid Atherosclerosis.
PLoS ONE 11(12): e0166918.

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  • Rabbit anti Human Delta-Like Protein 4
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP1274E, P, WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti Human Delta-like Protein 4 antibody detects human Delta-like protein 4 (DLL4), also known as Delta-4. DLL4 is a 685 amino acid single pass type 1 transmembrane glycoprotein of ~72kDa containing a single DSL domain and eight EGF-like domains. Human DLL4 is the homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the Notch receptor Notch1.

      DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formation of a differentiated vascular network (Mailhos et al. 2001). DLL4 has been found to be strongly expressed in tumour vessels of primary renal tumours (Patel et al. 2005) and bladder cancer (Patel et al. 2006), and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumour growth, with no apparent toxicity (Ridgway et al. 2006).

      Expression of DLL4 in normal human dermis is low in foetal tissues, becomes more intense during early life (0-20 years) and gradually declines thereafter as shown by immunohistochemical studies on FFPE tissues using rabbit anti human DLL4 antibody (Gunin et al. 2014).
    • Intended Use
    • Target Species
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      MouseExpected from Sequence
      RatExpected from Sequence
      ChimpanzeeExpected from Sequence
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to DLL4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
    • Immunogen
      Synthetic peptide corresponding to the internal region of human DLL4.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      blood circulation
      plasma membrane
      cell communication
      cell differentiation
      Notch binding
      integral to membrane
      Notch signaling pathway
      calcium ion binding
      Notch receptor processing
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Paraffin515ug/ml
      Western Blotting1/3001/1500

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Human ovary.
    • Immunofluorescence
    • Western Blotting
      AHP1274 detects a band of approximately 74kDa in pancreatic cell lysates.
    • Instructions For Use

    Additional Delta-Like Protein 4 Antibody Formats

    Formats Applications Sizes available
    Delta-Like Protein 4 Antibody : Purified E, P, WB 50 µg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Antigen Retrieval Buffer, pH8.0BUF025C50 mlP
          TidyBlot™ Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          Antigen Retrieval Buffer, pH8.0BUF025A500 mlP

          Recommended Positive Controls

            Histology Controls

              Human ovary.
              • Application NameReference Images
                Immunohistology - Paraffin


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