CD261 Antibody

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CD261 Antibody gallery image 1

Western blot analysis of HeLa whole cell lysate probed with Rabbit anti Human CD261 (AHP439)

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CD261 Antibody gallery image 2

Immunocytochemical staining of Jurkat cells with Rabbit anti Human CD261 (AHP439)

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  • Rabbit anti Human CD261 (C-Terminal)
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP439C, WBdatasheet pdfdatasheet pdf0.1 mg
    AHP439
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Rabbit anti Human CD261 antibody recognizes the ~57 kDa apoptosis related protein designated Death receptor 4, also known as CD261 or DR4. CD261 is a cell surface protein with a death domain that has functional similarities to TNFR1 and CD95. The ligand for DR-4 is TRAIL, and thus CD261 has also been called TRAIL-RI. CD261 is expressed in most human tissues. In contrast to CD95, TNFR1 and DR3 it does not use FADD to transmit death signals.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to CD261 (DR4) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
    • Preservative Stabilisers
      0.02%Sodium Azide
    • Immunogen
      Peptide from C-terminus of human CD261 (DR4)
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      death receptor activity
      TRAIL binding
      caspase activator activity
      activation of caspase activity
      induction of apoptosis via death domain receptors
      activation of NF-kappaB-inducing kinase activity
      transcription factor binding
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Immunohistology - Frozen
      Western Blotting1/5001/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD261 Antibody Formats

    Formats Applications Sizes available
    CD261 Antibody : Purified C, WB 0.1 mg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

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      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          TidyBlot Western Blot Detection Reagent:HRPSTAR209P0.5 mlWB*
          STAR209P

          Recommended Positive Controls

            Histology Controls

              Further Reading

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