CD14 Antibody | 61D3

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  • Mouse anti Human CD14:RPE-Cy5
  • Mouse anti Human CD14:RPE-Cy5.5
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Monoclonal Antibody
  • Clone
    61D3
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2804CFdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2804C
    MCA2804PECY5.5Fdatasheet pdfdatasheet pdf100 Tests/1ml
    MCA2804PECY5.5
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Human CD14 antibody, clone 61D3 recognizes human CD14, otherwise known as monocyte differentiation antigen. It is a ~40 kDa protein found on cell surfaces, particularly macrophages. CD14 acts as a co-receptor (along with the Toll-like receptor TLR 4 and MD-2) to mediate the innate immune response to bacterial lipopolysaccharide.
    • Intended Use
    • Target Species
      Human
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Bovineyes
      Dogyes
      Rabbityes
      Sheepyes
      Pigyes
      Cynomolgus monkeyyes
      Goatyes
      Catyes
      Minkyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG conjugated to R. Phycoerythrin (RPE) - Cy5 - liquid
      Purified IgG conjugated to R. Phycoerythrin - Cy5.5 (RPE-Cy5.5) - liquid
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
      0.1% Sodium Azide (NaN3)
      Sucrose
      0.1% Sodium Azide (NaN3)
      Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      6 months from date of despatch.
      6 months from date of despatch.
    • GO Terms
      inflammatory response
      lipoteichoic acid binding
      anchored to membrane
      apoptosis
      cellular response to lipopolysaccharide
      innate immune response
      Toll signaling pathway
      lipopolysaccharide binding
      positive regulation of tumor necrosis factor production
      phagocytosis
      opsonin receptor activity
      cellular response to lipoteichoic acid
      peptidoglycan receptor activity
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
      Cy® and CyDye® are registered trademarks of GE Healthcare
      Cy® and CyDye® are registered trademarks of GE Healthcare
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat
    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional CD14 Antibody Formats

    Formats Clone Applications Sizes available
    CD14 Antibody : RPE-CY5.5 61D3 F 100 Tests/1ml
    CD14 Antibody : RPE-Cy5 61D3 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A
          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Human SeroblockBUF070B200 TestF
          BUF070B
          Human SeroblockBUF070A50 TestF
          BUF070A

          Recommended Positive Controls

            Histology Controls

              References

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