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Carbonic Anhydrase II Antibody

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From: Doyle KP, Cekanaviciute E, Mamer LE, Buckwalter MS. TGFβ signaling in the brain increases with aging and signals to astrocytes and innate immune cells in the weeks after stroke.
J Neuroinflammation. 2010 Oct 11;7:62.

  • Carbonic Anhydrase II Antibody thumbnail image 1
  • Sheep anti Human Carbonic Anhydrase II
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP206ID, IF, WBdatasheet pdfdatasheet pdf1 ml
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Sheep anti Human carbonic anhydrase II antibody recognizes human carbonic anhydrase II, also known as Carbonate dehydratase II orCarbonic anhydrase C. Carbonic anhydrase II is a 259 amino acid ~30 kDa enzyme essential for bone resorption and osteoclast differrentiation.

      Mutations in the CA2 gene has been identified as the cause of Osteopetrosis, autosomal recessive 3 (OPTB3), a rare disease characterized by particularly dense bone, cerebral calcification and renal tubular acidisis (Shah et al 2004).

      Product identity is confirmed by double diffusion vs human CAII. No reactivity is seen in immunodiffusion against CAI.
    • Intended Use
    • Target Species
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to human carbonic anhydrase II were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
    • Preservative Stabilisers
      0.09%Sodium Azide
    • Immunogen
      Purified human carbonic anhydrase II (CAII) prepared from erythrocytes.
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Glycine buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      apical part of cell
      zinc ion binding
      carbonate dehydratase activity
      one-carbon metabolic process
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting1/1000

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Sheep anti human carbonic anyhdrase II recognises a band of ~18kDa in rat adrenomedullary chromaffin cell lysates, see Salman et al. for details.

      Loading control
      Rabbit anti Human β-actin (AHP1387)
    • Instructions For Use

    Additional Carbonic Anhydrase II Antibody Formats

    Formats Applications Sizes available
    Carbonic Anhydrase II Antibody : Purified ID, IF, WB 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Carbonic Anhydrase II Antibody thumbnail image 1
              • Application NameReference Images
                Western Blotting


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