APEX1 Antibody (PrecisionAbTM Antibody)

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APEX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) gallery image 1

Western blot analysis of whole cell lysates probed with APEX1 antibody followed by detection with HRP conjugated Donkey anti Sheep/Goat IgG (1/10,000, STAR88P) and visualized on the ChemiDoc MP with 22 second exposure. Arrow points to APEX1 (molecular weight 35 kDa)

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  • APEX1 Antibody (PrecisionAb<sup>TM</sup> Antibody) thumbnail image 1
  • Goat anti APEX1
  • APEX1 Antibody with Control Lysate
  • APEX1 Antibody with Control Lysate
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    APEX1 antibody recognizes DNA-(apurinic or apyrimidinic site) lyase, also known as APEX nuclease, APEN apurinic-apyrimidinic endonuclease 1, AP endonuclease 1, APE-1, REF-1 or redox factor-1. The antibody has been extensively validated for western blotting using whole cell lysates.
    • Product Type
      PrecisionAb™ Polyclonal
    • Isotype
      Polyclonal IgG
    • Format
      Purified
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      VPA00060WBdatasheet pdfdatasheet pdf100 µl
      VPA00060
      VPA00060KWBdatasheet pdfdatasheet pdf datasheet pdf10 Westerns
      VPA00060K
      VPA00060KTWBdatasheet pdfdatasheet pdf datasheet pdf2 Westerns
      VPA00060KT
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      References
      Reviews
      -
      • Goat anti Human APEX1 antibody recognizes apurinic/apyrimidinic endonuclease 1 (APE1), also known as redox effector factor-1 (Ref-1). APEX1 is a 35 kDa multifunctional protein that is ubiquitously expressed at high basal levels.

        APEX1 acts on apurinic/apyrimidinic (AP) sites in DNA as a major member of the base excision repair (BER) pathway and is involved in oxidative DNA damage repair. It is responsible for the maintenance of the cellular proliferative rate, and acts by modulating DNA binding activity of several transcription factors including NFkB, Egr-1, p53, AP-1, CREB, HIF-alpha and members of the Pax family. APEX1 has been shown to regulate hsp90 expression in thyroid cancer cells, in a novel tumorigenic pathway. APEX1 is also required in normal embryonic hematopoiesis.

        Goat anti Human APEX1 antibody detects APEX1 as a single band of 35 kDa in a wide range of human cell line lysates tested.
      • Target Species
        Human
      • Western Blotting
        Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
        Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
        Goat anti APEX1 detects a band of approximately 35 kDa in Jurkat cell lysates.
      • Product Form
        Purified IgG - liquid
        Purified IgG - liquid
        Purified IgG - liquid
      • Preparation
        Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 10 Westerns100μl Goat polyclonal antibody purified by affinity chromatography
        Pack Size: 2 Westerns20μl Goat polyclonal antibody purified by affinity chromatography
      • Buffer Solution
        TRIS buffered saline
        TRIS buffered saline
        TRIS buffered saline
      • Preservative Stabilisers
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
        0.02% Sodium Azide (NaN3)
        0.5% Bovine Serum Albumin
      • Immunogen
        Peptide with sequence PKRGKKGAVAEDGD-C from the N terminus of the protein sequence.
      • Instructions for Use
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
        Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
      • Lysate Composition
        400μg Jurkat lysate lyophilized in RIPA buffer.
        400μg Jurkat lysate lyophilized in RIPA buffer.
      • Lysate Reconstitution
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
        - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
        - If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
        Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
      • Storage
        Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
        Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

        Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
      • Shelf Life
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
        As supplied, 12 months from date of despatch.
      • Acknowledgements
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
        PrecisionAb™ is a trademark of Bio-Rad Laboratories.
      • Regulatory
        For research purposes only
      • GO Terms
        base-excision repair
        oxidoreductase activity
        ribonuclease H activity
        positive regulation of DNA repair
        DNA recombination
        metal ion binding
        ribosome
        positive regulation of anti-apoptosis
        transcription corepressor activity
        mitochondrion
        transcription coactivator activity
        3'-5' exonuclease activity
        chromatin DNA binding
        centrosome
        DNA-(apurinic or apyrimidinic site) lyase activity
        regulation of mRNA stability
        nuclear speck
        site-specific endodeoxyribonuclease activity, specific for altered base
        damaged DNA binding
        nucleolus
        perinuclear region of cytoplasm
        endoplasmic reticulum
        RNA binding
        protein binding
        phosphodiesterase I activity
        uracil DNA N-glycosylase activity
      • UniProt
      • Entrez Gene
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000
      • Application NameYesNoMin DilutionMax Dilution
        Western Blotting1/1000

      • PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
      • Copyright © 2016 Bio-Rad

      Recommended Secondary Antibody

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P
        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
        STAR88P

        Recommended Negative Isotype Control

          Useful Reagents

            Recommended Positive Controls

              Histology Controls

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