Alkaline Phosphatase Antibody

//www.bio-rad-antibodies.com/human-alkaline-phosphatase-antibody-0300-1909.html?RenderPageSpecific=1
Alkaline Phosphatase Antibody gallery image 1

Published customer image:
Sheep anti Human alkaline phosphatase antibody used for the detection of alkaline phosphatase in Caco-2/TC7 cells by immunoelectron microscopy.
Image caption:
PPM supply induces movement of SR-BI/CLA-1 towards raft microdomains. (A) Caco-2/TC7 cells were harvested in the presence of Triton X-100 and the lysate fractionated on a 5–40% sucrose gradient. Eleven fractions were collected for immunoblots of SR-BI/CLA-1, EEA1 (early endosome antigen 1) and flottilin-1 (raft marker). (B) Caco-2/TC7 cells were cultured in the absence (control) or presence of PPM or IPM for 10 min and then harvested in the presence of Triton X-100. Cell lysates were applied to a 5–40% sucrose gradient and eleven fractions collected. Fractions 3 to 8 were analyzed by immunoblotting with antibodies against SR-BI/CLA-1 (left panel) and flottilin-1 (right panel). (C) Immunolocalization of SR-BI/CLA-1 and alkaline phosphatase (PLAP, used as raft marker) in the brush border of Caco-2/TC7 cells supplied with PPM. SR-BI/CLA-1 is labelled with anti-rabbit immunoglobulin-gold complexes (18-nm particles) and PLAP with anti-sheep immunoglobulin-gold complexes (12-nm particles). MV, microvilli; bar, 100 nm.

From: Béaslas O, Cueille C, Delers F, Chateau D, Chambaz J, Rousset M, et al. (2009) Sensing of Dietary Lipids by Enterocytes: A New Role for SR-BI/CLA-1.
PLoS ONE 4(1): e4278.

Enlarge
Alkaline Phosphatase Antibody gallery image 2

Published customer image:
Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in human muscle derived cells (mdcs) by flowcytometry.
Image caption:
Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar ?=?50 µm. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI (blue). Scale bar ?=?50 µm. g. Representative FACS images showing the expression of PDGFRß, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparations

From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011) Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.

Enlarge
Alkaline Phosphatase Antibody gallery image 3

Published customer image:
Sheep anti Human alkaline phosphatase antibody used for the evaluation of alkaline phosphatase expression in pD2 cells by flowcytometry.
Image caption:
Characterization and phenotype of human mdcs. a. Cultured human mdcs consisted of small, triangular adherent cells and small, round floating cells. Scale bar ?=?50 µm. b. Proliferation curve of one normal human mdc preparation (pN1) and one DMD mdc preparation (pD1). The average mean population doubling time for these cells is 33.79±1.24 hrs. c, d, e, f. Representative images showing the expression of NG2 (c, green), CD49b (d, green), CD29 (e, green) and CD44 (f, green) by pN1 human mdcs. Nuclei were counterstained with 10 µg/ml DAPI (blue). Scale bar ?=?50 µm. g. Representative FACS images showing the expression of PDGFRß, ALP, CD49b, CD29, CD71, CD90, Stro-1, CD56 and CD34 by human mdcs. Isotype control is shown in red in each image, and positive signal is shown in green. Note that most of these markers, apart from PDGFRβ and CD56, were expressed at similar levels among cell preparations

From: Meng J, Adkin CF, Xu S-w, Muntoni F, Morgan JE (2011) Contribution of Human Muscle-Derived Cells to Skeletal Muscle Regeneration in Dystrophic Host Mice.
PLoS ONE 6(3): e17454.

Enlarge
  • Alkaline Phosphatase Antibody thumbnail image 1
  • Alkaline Phosphatase Antibody thumbnail image 2
  • Alkaline Phosphatase Antibody thumbnail image 3
  • Sheep anti Human Alkaline Phosphatase
(Rated 0.0 out of 5 based on 0 customer reviews)
  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    0300-1909E, EMdatasheet pdfdatasheet pdf1 ml
    0300-1909
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Sheep anti Human Alkaline Phosphatase antibody recognizes alkaline phosphatase, it has been found to be reactive with both placental and bone alkaline phosphatase.
    • Intended Use
    • Target Species
      Human
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Purified alkaline phosphatatse from human placenta.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 5.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • GO Terms
      integral to membrane
      alkaline phosphatase activity
      anchored to membrane
      cell surface
      metal ion binding
      plasma membrane
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immuno-electron Microscopy

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional Alkaline Phosphatase Antibody Formats

    Formats Applications Sizes available
    Alkaline Phosphatase Antibody : Purified E, EM 1 ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

              Write your review

              You may also be interested in...