AIF antibody
Rabbit anti AIF (aa517-531)
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- AIF
- Region
- (aa517-531)
Rabbit anti Human AIF antibody recognizes human Apoptosis-inducing factor 1, mitochondrial, also known as Programmed cell death protein 8 or apoptosis inducing factor (AIF). AIF is a 613 amino acid member of the FAD-dependent oxidoreductase family.Rabbit anti Human AIF antibody reacts with an internal region of AIF (within the region aa 517-531. AIF is widely expressed in human tissues and localizes in the mitochondria. AIF translocates to the nucleus upon induction of apoptosis. Multiple isoforms of AIF are generated by alternative splicing. Rabbit anti Human AIF antibody recognizes isoforms 1, 2,3 and 5. It is not expected to recognize either isoforms 4 or 6 as they lack the immunogen sequence. Mutations in the AIFM1 can result in various disease conditions including COXPD6 and Cowchock syndrome. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antisera to AIF were raised by repeated immunisation of rabbit with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.02% Sodium Azide - Immunogen
- Peptide corresponding to amino acids 517 - 531 of human AIF.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Immunohistology - Paraffin 1 | 10 ug/ml | ||
Western Blotting | 0.25ug/ml | 1ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
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Further Reading
-
Susin. S.A. et al. (1999) Molecular characterization of mitochondrial apoptosis - inducing factor.
Nature. 397: 441-6.
AHP578
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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