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CD45 antibody | IH-1

Product Code Applications Pack Size List Price Your Price Qty
MCA1130
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.2 mg loader
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loader

Mouse anti Guinea Pig CD45 antibody, clone IH-1 reacts with CD45 on all leucocytes.

Mouse anti Guinea Pig CD45 antibody, clone IH-1 immunoprecipitates proteins of M.Wt. 220,000, 210,000 and 195,000 and 180,000 from lymph node lymphocytes and proteins of M.Wt 195,000, 180,000 and 170,000 from thymocytes.

Target Species
Guinea Pig
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
<0.1% sodium azide (NaN3)
Immunogen
Guinea pig leukemic B lymphocyte L2C cells.
Approx. Protein Concentrations
IgG concentration 1mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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References for CD45 antibody

  1. Hart, I.J. et al. (1992) Subpopulations of guinea-pig T lymphocytes defined by isoforms of the leucocyte common antigen.
    Immunology. 77 (3): 377-84.
  2. Wang, Y. et al. (2010) Local host response to chlamydial urethral infection in male guinea pigs.
    Infect Immun. 78: 1670-81.
  3. Lacy, H.M. et al. (2011) Essential role for neutrophils in pathogenesis and adaptive immunity in Chlamydia caviae ocular infections.
    Infect Immun. 79 (5): 1889-97.
  4. Ishikawa, M. et al. (2017) Transplantation of neurons derived from human iPS cells cultured on collagen matrix into guinea-pig cochleae.
    J Tissue Eng Regen Med. 11 (6): 1766-78.
  5. Tura-Ceide, O. et al. (2017) Cigarette smoke challenges bone marrow mesenchymal stem cell capacities in guinea pig.
    Respir Res. 18 (1): 50.

Flow Cytometry

RRID
AB_321724

MCA1130

160669 160818 165562

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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