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Green Fluorescent Protein Antibody

Green Fluorescent Protein Antibody gallery image 1

Western blot analysis of recombinant RFP ( lane 1 & 2), recombinant YFP (lane 3 & 4) and recombinant EGFP (lane 5 & 6) probed with Rabbit anti GFP antibody

  • Green Fluorescent Protein Antibody thumbnail image 1
  • Rabbit anti Green Fluorescent Protein
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AHP2984WBdatasheet pdfdatasheet pdf50 µg
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    • Rabbit anti GFP antibody recognizes green fluorescent protein (GFP) and its variants. GFP is a ~27 kDa protein derived from the jellyfish Aequorea victoria. GFP fluoresces green (509 nm) when excited by blue light (395 nm), and is commonly used as a marker of gene expression. Rabbit anti GFP antibody is known to cross-react with enhanced green fluorescent protein (EGFP), yellow fluorescent protein (YFP) and red fluorescent protein (RFP).
    • Intended Use
    • Product Form
      Purified IgG - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antiserum to green fluorescent protein (GFP) was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
    • Preservative Stabilisers
      30% Glycerol
      0.5% Bovine Serum Albumin
      0.03% ProclinTM 300
    • Immunogen
      E. coli expressed recombinant EGFP
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 0.5 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Store at -20oC
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch
    • Acknowledgements
      ProclinTM 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Western Blotting0.54.0 ug/ml

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
      Rabbit anti GFP antibody recognizes a band of approximately 30-37 kDa on recombinant enhanced green fluorescent protein (EGFP).
      Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant red fluorescent protein (RFP).
      Rabbit anti GFP antibody recognizes a band of approximately 32-34 kDa on recombinant yellow fluorescent protein (YFP).
    • Instructions For Use

    Additional Green Fluorescent Protein Antibody Formats

    Formats Applications Sizes available
    Green Fluorescent Protein Antibody : Purified WB 50 µg
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Rabbit IgG (Fc):BiotinSTAR121B1 mgE, WB
      Sheep anti Rabbit IgG:Biotin2AB02B1 mlC, E, P, WB
      Sheep anti Rabbit IgG:DyLight®488STAR36D488GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:DyLight®549STAR36D549GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®649STAR36D649GA0.1 mgF, IF
      Sheep anti Rabbit IgG:DyLight®680STAR36D680GA0.1 mgF, IF, WB
      Sheep anti Rabbit IgG:Dylight®800STAR36D800GA0.1 mgF, IF, WB
      Goat anti Rabbit IgG (Fc):FITCSTAR121F1 mgF
      Sheep anti Rabbit IgG:FITCSTAR34B1 mgC, F
      Goat anti Rabbit IgG (Fc):HRPSTAR121P1 mgE, WB
      Goat anti Rabbit IgG (H/L):HRPSTAR124P1 mgC, E, WB
      Sheep anti Rabbit IgG:HRP (Multi Adsorbed)STAR541 mgC, E, WB
      Sheep anti Rabbit IgG:RPESTAR35A1 mlF

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          Rabbit anti mCherryAHP232650 µgWB
          Rabbit anti Yellow Fluorescent ProteinAHP298350 µgWB
          Rabbit anti Blue Fluorescent ProteinAHP298550 µgWB
          Rabbit anti Cyan Fluorescent ProteinAHP298650 µgWB
          Rabbit anti Red Fluorescent ProteinAHP298750 µgWB
          Mouse anti mCherryMCA602050 µgWB

          Recommended Positive Controls

            Histology Controls

                • Product Images

                • Green Fluorescent Protein Antibody thumbnail image 1

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