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Rhizopus arrhizus Antibody | WSSA-RA-1

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Rhizopus arrhizus Antibody | WSSA-RA-1 gallery image 1

Renal tissue presenting zygomycosis stained with Mouse anti Rhizopus Arrhizus (MCA2577)

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  • Mouse anti Rhizopus arrhizus

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  • Product Type
    Monoclonal Antibody
  • Clone
    WSSA-RA-1
  • Isotype
    IgM
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2577E, P *, WBdatasheet pdfdatasheet pdf0.25 mg
    MCA2577
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Mouse anti Rhizopus arrhizus antibody, clone WSSA-RA-1 recognizes Rhizopus arrhizus and other members of the family Mucoraceae including Absidia corymbifera and Rhizomucor pusillus, reacting strongly with the cytoplasm of hyphae and also possibly with the walls and septae, where present.

      R. arrhizus, an angio-invasive filamentous fungus, is one of the main causative agents of systemic bovine and human zygomycosis, a worldwide and often fatal respiratory disease. Clone WSSA-RA-1 has been successfully used in immunohistochemistry for the specific and consistent in situ diagnosis of systemic bovine zygomycosis, attributed to its possible binding to a highly glycosylated moiety on non-structural components.

      Clone WSSA-RA-1 does not bind to water-soluble somatic antigens (WSSA) of Aspergillus spp.
    • Intended Use
    • Target Species
      Fungal
    • Product Form
      Purified IgM - liquid
    • Reconstitution
    • Preparation
      Purified IgM prepared by ammonium sulphate precipitation from tissue culture supernatant.
    • Preservative Stabilisers
      0.09%Sodium Azide (NaN3)
    • Immunogen
      Water-soluble somatic antigens (WSSA) from Rhizopus arrhizus.
    • Purity
    • Approx. Protein Concentrations
      IgM concentration 1.0mg/ml.
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline.
    • Fusion Partners
      Spleen cells from immunised Balb/c ABom mice were fused with cells of the X63-Ag8.653 myeloma cell line.
    • Storage
      Store at +4oC or at -20oC if preferred.
      Storage in frost-free freezers is not recommended.
      This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA
      Immunoprecipitation
      Western Blotting
      Immunohistology - Paraffin(1)1/50
      (1)
      This product requires protein digestion pre-treatment of paraffin sections e.g. See Jensen et al. (2000) for details.

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
      Lymph nodes from R.arrhizus infected cattle.
    • Immunofluorescence
    • Western Blotting
      Mouse anti Rhizopus arrhizus detects a number diffuse band/s of between ~14-110kDa of Rhizopus arrhizus water-soluble somatic antigens (Jensen et al. 1996).
    • Instructions For Use

    Additional Rhizopus arrhizus Antibody Formats

    Formats Clone Applications Sizes available
    Rhizopus arrhizus Antibody : Purified WSSA-RA-1 E, P *, WB 0.25 mg
    • Copyright © 2017 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Goat anti Mouse IgG/A/M:Alk. Phos.STAR87A1 mgC, E, WB
      STAR87A
      Goat anti Mouse IgM:Alk. Phos.(Human Adsorbed)STAR138A1 mlC, E, P, WB
      STAR138A
      Human anti Mouse IgM:FITCHCA040F0.1 mgF
      HCA040F
      Human anti Mouse IgM:HRPHCA040P0.1 mgE
      HCA040P
      Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)STAR87P1 mgE
      STAR87P
      Goat anti Mouse IgM:RPESTAR86PE0.5 mlF
      STAR86PE

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              Lymph nodes from R.arrhizus infected cattle.
              • Application NameReference Images
                Immunohistology - Paraffin

              References

              1. Jensen, H.E. et al. (1996) Immunohistochemical diagnosis of systemic bovine zygomycosis by murine monoclonal antibodies.
              Vet. Pathol. 33: 176-183.
              2. Jensen, H.E. et al. (1996) Diagnosis of systemic mycoses by specific immunohistochemical tests.
              APMIS. 104: 241-258.
              3. Jensen, H.E. et al. (1997) The use of immunohistochemistry to improve sensitivity and specificity in the diagnosis of systemic mycoses in patients with haematological malignancies.
              J. Pathol. 181: 100-105.
              4. Jensen, H.E. et al. (1996) Development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis.
              J. Vet. Diagn. Invest. 8: 68-75.
              5. Arendrup, M.C. et al. (2009) Breakthrough Aspergillus fumigatus and Candida albicans double infection during caspofungin treatment: laboratory characteristics and implication for susceptibility testing.
              Antimicrob Agents Chemother. 53: 1185-93.
              6. Yasuda, M. et al (2012) A case of intestinal mucormycosis in a common marmoset (Callithrix jacchus).
              J Vet Med Sci. 74: 357-9.
              7. Galiza G.J.N. et al. (2014) Usage of three immunohistochemical methods in the detection of aspergillosis and zygomycosis in animals.
              Pesquisa Veterinária Brasileira. 34 (7): 637-642.
              8. Suzuta F et al. (2015) Variations in the morphology of Rhizomucor pusillus in granulomatous lesions of a Magellanic penguin (Spheniscus magellanicus).
              J Vet Med Sci. 77 (8): 1029-31.
              9. Nishimura, M. et al. (2014) Zygomycotic mediastinal lymphadenitis in beef cattle with ruminal tympany.
              J Vet Med Sci. 76 (1): 123-7.
              10. Ogasawara, F. et al. (2016) Concurrent Fowlpox and Candidiasis Diseases in Backyard Chickens with Unusual Pox Lesions in the Bursa of Fabricius.
              Avian Dis. 60 (3): 705-8.

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