Flow cytometry antibodies, kits, reagents

Chapter 2: Principles of Fluorescence

Why Use a Fluorescent Marker?

The purpose of a fluorescent marker, such as a fluorophore-conjugated antibody, is to directly target an epitope of interest and to allow its biological and biochemical properties to be measured. Fluorescent markers are useful in a wide range of applications, including identifying and quantifying distinct populations of cells, cell surface receptors, or intracellular organelles; cell sorting; immunophenotyping; calcium influx experiments; determining nucleic acid content; measuring enzyme activity; and for apoptosis studies.

Several fluorophores can be excited by a single laser. And by using filters to segregate the light to the proper detectors and using more than one fluorophore, it is possible to analyze several parameters of the sample at any one time. This forms the basis of multicolor fluorescence studies.