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Epidermal growth factor receptor (EGF R), a transmembrane glycoprotein, is a member of the ErbB receptor tyrosine kinase family. Upon ligand binding, EGF R undergoes dimerization. Specific tyrosine residues in the cytoplasmic domain are subsequently phosphorylated, and adaptor proteins and enzymes are recruited to transmit signals from EGF R via signaling pathways to the nucleus. This regulates diverse biological functions like cell proliferation, differentiation, migration, and apoptosis (Morandell et al. 2008; Yarden and Sliwkowski 2001).
An important signaling pathway downstream of EGF R is RAS/RAF. The adaptor protein growth factor receptor-bound protein 2 (Grb2) complexes with son of sevenless (SOS) associating with EGF R, either directly or through binding to SHC-transforming protein 1 (SHC1) (Waters et al. 1996). SOS mediates the activation of RAS proteins (Downward 1996). Activated RAS recruits and activates RAF family kinases promoting activation of extracellular signal-regulated kinase (ERK) mediated through mitogen activated protein kinase 1 (MEK). Activated kinases move to the nucleus and regulate transcription factors involved in cell proliferation (Liebmann 2001).
The PI3K/AKT cascade is another important target in EGF R signaling (Stover et al.1995). Activated phosphoinositide 3-kinase (PI3K) phosphorylates and activates protein kinase B (AKT), leading to phosphorylation of mammalian target of rapamycin (mTOR). Subsequent phosphorylation of ribosomal protein S6 kinase beta-1 (P70S6K) promotes protein synthesis.
ErbB family members play an important role in the development and malignancy of various human cancers (Mishra et al. 2017). Kinase mutations and/or overexpression of the receptor can result in deregulation in signaling networks; understanding the precise roles of the EGF R-mediated signaling pathways is crucial for translating these findings into new cancer therapies.
We have compiled key proteins involved in the EGF R signaling pathway into one handy poster you can download for your reference.
Browse our range of antibodies against targets involved with EGF R signaling below. Please use the filters to sort the attributes in the table below in order to find the antibody that fits your exact requirements. If you need any further assistance please do not hesitate to contact us.