CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9

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CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9 gallery image 1

Canine CD3 positive cells from peripheral blood analysed for CD4 and CD8 staining using anti Dog CD3:FITC/CD4:PE/CD8:Alexa Fluor®647 (TC014)

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CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9 gallery image 2

FITC conjugated Mouse anti Canine CD3, PE conjugated Rat anti Canine CD4 and A647 conjugated Rat anti Canine CD8 (TC014). All experiments performed on red cell lysed canine blood gated on mononuclear cells.

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  • CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9 thumbnail image 1
  • CD3/CD4/CD8 | CA17.2A12 / YKIX302.9 / YCATE55.9 thumbnail image 2
  • anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647
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  • Product Type
    Triple Color Reagent
  • Clone
    CA17.2A12 / YKIX302.9 / YCATE55.9
  • Isotype
    Cocktail
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    TC014Fdatasheet pdfdatasheet pdf100 Tests/1ml
    TC014
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Anti Dog CD3:FITC/CD4:RPE/CD8:Alexa Fluor® 647 is a triple color reagent recognizing canine CD3, CD4 and CD8 cell surface antigens.

      Clone CA17.2A12 recognizes canine CD3, which is expressed by T lymphocytes. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE 55.9 recognizes CD8 alpha, which is expressed by cytotoxic T cells.
    • Intended Use
    • Target Species
      Dog
    • Product Form
      Triple Color combination consisting of FITC, RPE, Alexa Fluor® 647 conjugated monoclonal antibodies mixed in optimal ratio - lyophilized
    • Reconstitution
      Reconstitute with 1.0 ml distilled water
    • Preparation
    • Preservative Stabilisers
      0.09%Sodium Azide
      1%Bovine Serum Albumin
      5%Sucrose
    • Purity
    • Approx. Protein Concentrations
    • Antibody Isotypes
      CD3 FITC reagent: Mouse IgG1
      CD4 RPE reagent: Rat IgG2a
      CD8 Alexa Fluor® 647 reagent: Rat IgG1
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
    • Storage
      Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.

      DO NOT FREEZE.

      This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of reconstitution
    • GO Terms
      transmembrane receptor activity
      immune response
      cell adhesion
      integral to membrane
      T cell selection
    • UniProt
    • Entrez Gene
    • Acknowledgements
      This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow CytometryNeat

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Recommended Protocol
    • Flow Cytometry
      Use 10ul of the suggested working dilution to label 106 cells in 100ul
    • ELISA
    • Immunohistology
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Western Blotting
    • Instructions For Use

    Additional CD3/CD4/CD8 Formats

    Formats Clone Applications Sizes available
    CD3/CD4/CD8 : 3 Color CA17.2A12 / YKIX302.9 / YCATE55.9 F 100 Tests/1ml
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        DescriptionProduct CodePack SizeApplicationsList PriceQuantity
        Mse IgG1:FITC/Rat IgG2a:RPE/Rat IgG1:Alexa Fluor® 647 -ve ControlTC023100 Tests/1mlF *
        TC023

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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