Red Cell Lysing Buffer

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  • Product Type
    Accessory Reagent
1 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    BUF04BFdatasheet pdfdatasheet pdf100 Test/20ml
    BUF04B
    BUF04CFdatasheet pdfdatasheet pdf500 Tests/100ml
    BUF04C
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Intended Use
    • Product Form
    • Reconstitution
    • Preparation
    • Preservative Stabilisers
    • Purity
    • Approx. Protein Concentrations
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
    • Storage
      The 10X concentrated ERYTHROLYSE should be stored at room temperature and is stable for 1 year. Do not use if any discolouration or precipitation occurs in the ERYTHROLYSE solution. The information regarding the products is believed to be accurate, but the information and products have no warranty or guarantee other than those specified, since the ultimate conditions of use and the variability of the materials are beyond our control. We cannot be responsible for and we do not intend these products or the accompanying information to be used for patent infringement or other such violations
      The 10X concentrated ERYTHROLYSE should be stored at room temperature and is stable for 1 year. Do not use if any discolouration or precipitation occurs in the ERYTHROLYSE solution. The information regarding the products is believed to be accurate, but the information and products have no warranty or guarantee other than those specified, since the ultimate conditions of use and the variability of the materials are beyond our control. We cannot be responsible for and we do not intend these products or the accompanying information to be used for patent infringement or other such violations
    • Shelf Life
      1 year from the date of despatch.
      1 year from the date of despatch.
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry

    • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
      Bio-Rad ERYTHROLYSE is supplied as a 10X concentration solution which must be diluted 1/10 with distilled water before use.

      Bio-Rad ERYTHROLYSE is designed for use in whole blood immunofluorescent staining procedures and is suitable for use with human, rat and mouse blood.

      Following staining according to standard procedures (worksheets available on request), 2ml of diluted ERYTHROLYSE is added to 100ul of whole blood, mixed well and incubated for 10 minutes at room temperature. The tubes should then be centrifuged (400g, 5 minutes), the supernatant decanted and cells washed once in 2ml PBS/1% BSA before analysis by flow cytometry.
    • Instructions For Use
      Bio-Rad ERYTHROLYSE is supplied as a 10X concentration solution which must be diluted 1/10 with distilled water before use.

      Bio-Rad ERYTHROLYSE is designed for use in whole blood immunofluorescent staining procedures and is suitable for use with human, rat and mouse blood.

      Following staining according to standard procedures (worksheets available on request), 2ml of diluted ERYTHROLYSE is added to 100ul of whole blood, mixed well and incubated for 10 minutes at room temperature. The tubes should then be centrifuged (400g, 5 minutes), the supernatant decanted and cells washed once in 2ml PBS/1% BSA before analysis by flow cytometry.

    Additional Red Cell Lysing Buffer Formats

    Formats Applications Sizes available
    Red Cell Lysing Buffer : Reagent F 100 Test/20ml | 500 Tests/100ml
    • Copyright © 2016 Bio-Rad

    Recommended Secondary Antibody

      Recommended Negative Isotype Control

        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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