IL-10 Antibody | CC320

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IL-10 Antibody | CC320 gallery image 1

Published customer image:
Biotinylated Mouse anti Bovine interleukin-10 antibody, clone CC320 used as a detection reagent for the determination of IL-10 levels in ovine plasma by ELISA
Image caption:
Comparison of plasma concentrations of (A) IL-10 and (B) IL-6 measured over time in arthritic sheep treated and untreated with mesenchymal precursor cells (MPC). Each point represents the mean ± SEM of 7–8 sheep.**** Indicates a statistically significant difference between groups at that time point, p = 0.0001.

From: Dooley LM, Abdalmula A, Washington EA, Kaufman C, Tudor EM, Ghosh P, et al. (2015) Effect of Mesenchymal Precursor Cells on the Systemic Inflammatory Response and Endothelial Dysfunction in an Ovine Model of Collagen-Induced Arthritis.
PLoS ONE 10(5): e0124144.

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  • Mouse anti Bovine Interleukin-10:Low Endotoxin
  • Mouse anti Bovine Interleukin-10:Biotin
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  • Product Type
    Monoclonal Antibody
  • Clone
    CC320
  • Isotype
    IgG1
2 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    MCA2111BEdatasheet pdfdatasheet pdf0.25 mg
    MCA2111B
    MCA2111ELE, F *, FNdatasheet pdfdatasheet pdf0.5 mg
    MCA2111EL
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
    -
    • Mouse anti Bovine Interleukin-10 antibody, clone CC320 recognizes bovine IL-10.

      Mouse anti Bovine Interleukin-10 antibody, clone CC320 has been shown to neutralize the activity of bovine IL-10 as measured by the inhibition of the inhibitory activity of IL-10 on IFN gamma synthesis (Buza et al. 2004).
    • Intended Use
    • Target Species
      Bovine
    • Species Cross-Reactivity
      Target SpeciesCross Reactivity
      Horseyes
      Sheepyes
      Goatyes
      N.B. Antibody reactivity and working conditions may vary between species.
    • Product Form
      Purified IgG - liquid
      Purified IgG conjugated to Biotin - liquid
    • Reconstitution
    • Preparation
      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
      Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
    • Preservative Stabilisers
      None present
      0.09%Sodium Azide
    • Immunogen
      Plasmid cDNA encoding bovine IL-10.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
    • Fusion Partners
      Spleen cells from immunised Balb/c mice were fused with cells of the mouse sp2/0 myeloma cell line.
    • Storage
      Store at -20oC only.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC or at -20oC if preferred.

      This product should be stored undiluted.

      Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

      Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      18 months from date of despatch.
      18 months from date of despatch.
    • GO Terms
      positive regulation of gene-specific transcription
      negative regulation of nitric oxide biosynthetic process
      negative regulation of cytokine secretion involved in immune response
      inflammatory response
      positive regulation of cytokine secretion
      positive regulation of B cell apoptosis
      cytokine activity
      extracellular space
      receptor biosynthetic process
      interferon-gamma biosynthetic process
      negative regulation of membrane protein ectodomain proteolysis
      response to glucocorticoid stimulus
      negative regulation of interferon-gamma biosynthetic process
      negative regulation of B cell proliferation
      positive regulation of transcription factor activity
      immune response
      response to molecule of bacterial origin
      negative regulation of interleukin-6 production
    • UniProt
    • Entrez Gene
    • Acknowledgements
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA5ug/ml10ug/ml
      Functional Assays
      Flow Cytometry(1)
      (1)
      Membrane permeabilization is required for this application. Bio-Rad recommends the use of Leucoperm (BUF09) for this purpose.
    • Application NameYesNoMin DilutionMax Dilution
      ELISA2ug/ml5ug/ml

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
      This biotin conjugate may be used as detection reagent in a sandwich ELISA assay for bovine IL-10 with MCA2110 as capture reagent, see Bannerman, D.D.et al.
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use

    Additional IL-10 Antibody Formats

    Formats Clone Applications Sizes available
    IL-10 Antibody : Low Endotoxin CC320 E, F *, FN 0.5 mg
    IL-10 Antibody : Biotin CC320 E 0.25 mg
    • Copyright © 2016 Bio-Rad

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      Recommended Negative Isotype Control

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        Useful Reagents

          Recommended Positive Controls

            Histology Controls

              References

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