IgG Antibody

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IgG Antibody gallery image 1

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Sheep anti Bovine IgG polyclonal antibody used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP-specific Ig titres following primary and boost immunisations with TNP-conjugated antigens. Five calves were subcutaneously immunised with 3 mg TNP-CGG or TNP-AECM-FICOLL in QuilA then boosted 29 days later with the same immunising antigens. The dotted vertical line indicates when the booster TNP-CGG/TNP-AECM-FICOLL immunisations were given (day 29 pi). Blood samples were taken at various time-points following these injections and TNP-specific IgG (diamond symbols) and IgM (square symbols) titres were determined by ELISA in calves from the T-D group (open symbols) or T-I group (filled symbols). Results are expressed as the mean of determinations from individual calves in each group?±?SEM, n?=?5.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

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IgG Antibody gallery image 2

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Sheep anti Bovine IgG polyclonal antibody used for the the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
TNP-specific plasma and memory B cell detection post-boost immunisation with TNP-CGG (T-D group). Five calves were subcutaneously immunised with 3 mg TNP-CGG in QuilA then boosted 29 days later with the same immunising antigen. The dotted vertical line indicates when the booster TNP-CGG immunisation was given (day 29 pi). Blood samples were taken at various time-points following the immunisations. TNP-specific IgG, plasma cell (filled circles, left-axis) and memory B cell (open circles, right-axis) numbers were determined by B cell ELISPOT. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group, n?=?5).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

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IgG Antibody gallery image 3

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Sheep anti Bovine IgG polyclonal antibody used for the the enumeration of antibody secreting cells in Water Buffalo blood samples by ELISPOT assay.
Image caption:
Kinetics of antigen-specific IgG secreting plasma cells and memory B cells post-TTC immunisation. At day 134 post-primary TNP-conjugated immunisation, all calves were subcutaneously immunised with 3 mg TTC in IFA then boosted 63 days later with the same immunising antigen. Blood samples were taken at various time-points following these immunisations. The time of primary and boost TTC immunisations are indicated by vertical dotted lines (day 134 and 197 pi, respectively). Antigen-specific IgG, plasma cell (A) and memory B cell (B) numbers were determined by B cell ELISPOT and are identified as follows: CGG-specific (open triangle), TTC-specific (crosses) and TNP-specific (closed triangle) IgG secreting ASCs. Results were expressed as ASC per 106 PBMC for plasma cells and induced ASC per 106 cultured PBMC for memory B cells (mean of determinations from individual calves in each group for the TNP- and CGG-specific responses, n?=?5, and mean of determinations from all calves for the TTC-specific responses, n?=?10). a?=?p?<?0.05, b?=?p?<?0.001.

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

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IgG Antibody gallery image 4

Published customer image:
Sheep anti Bovine IgG polyclonal antibody used for the the evaluation of serum IgG levels in Water Buffaloes by ELISA.
Image caption:
Kinetics of TNP- and TTC-specific IgG titres following primary and boost TTC immunisations. TNP-specific IgG titres in calves from the T-D group (open diamonds) or T-I group (filled diamonds) and TTC-specific IgG titres in all calves (cross symbols) were determined by ELISA. The dotted vertical lines indicate when the primary and boost TTC immunisations were administered (days 134 and 197 pi, respectively). Results are expressed as the mean of determinations from individual calves in each group for the TNP-specific responses (n?=?5) and the mean of determinations from all calves for the TTC-specific responses (n?=?10).

From: Grant CF, Lefevre EA, Carr BV, Prentice H, Gubbins S, Pollard AJ, Charreyre C, Charleston B. Assessment of T-dependent and T-independent immune responses in cattle using a B cell ELISPOT assay. Vet Res. 2012 Oct 10;43:68.

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IgG Antibody gallery image 5

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Sheep anti Bovine IgG polyclonal antibody used for the measurement of cattle IgG resopnses totsetse fly antigens by ELISA.
Image caption:
Cattle IgG responses against WSE and, Tsal152–75 and Tsal1145–166 peptides. The IgG responses directed against whole saliva extracts (WSE) and the two candidate synthetic peptides were investigated in 43 animals from a tsetse infested area, 17 animals from a tsetse free area and four animals exposed experimentally to A. variegatum, An. gambiae, Tabanidae spp. or Stomoxys spp. (other arthropods). Individual =ΔOD values are represented by empty circles. In the scatter plot, the horizontal bars indicate the median value for each group. Statistical significance between the different groups is indicated (non-parametric Mann–Whitney U-test)

From: Somda MB, Cornelie S, Bengaly Z, Mathieu-Daudé F, Poinsignon A, Dama E, Bouyer J, Sidibé I, Demettre E, Seveno M, Remoué F, Sanon A, Bucheton B.
Identification of a Tsal152-75 salivary synthetic peptide to monitor cattle exposure to tsetse flies.
Parasit Vectors. 2016 Mar 15;9(1):149.

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IgG Antibody gallery image 6

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Biotin conjugated Sheep anti Bovine IgG antibody (AAI23B) used for the detection of M. avium subsp. paratuberculosis protein specific IgG secreted by cells from infected calves by ELISPOT along with biotinylated Sheep anti Bovine IgA antibody (AAI20B) used to evaluate similar IgA responses.
Image caption:
Net IgA-ASC and IgG-ASC responses to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in mid-jejunal segments and assayed for IgA (A) and IgG (B) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. Cell suspensions were prepared from PP collected from M. avium subsp. paratuberculosis-infected and uninfected compartments in terminal small intestinal segments and assayed for IgG (C) ASCs specific to recombinant M. avium subsp. paratuberculosis proteins. The net number of ASC specific to each M. avium subsp. paratuberculosis protein in infected compartments was calculated using the formula provided in Materials and Methods. M. avium subsp. paratuberculosis proteins for which all calves (n = 3) had detectable net ASC responses are shown with significant (P <0.05) increases in the frequency of M. avium subsp. paratuberculosis-protein specific ASCs denoted by an asterisk. Data presented are mean values with SEM.

From: Facciuolo A, Gonzalez-Cano P, Napper S, Griebel PJ, Mutharia LM (2016)
Marked Differences in Mucosal Immune Responses Induced in Ileal versus Jejunal Peyer’s Patches to Mycobacterium avium subsp. paratuberculosis Secreted Proteins following Targeted Enteric Infection in Young Calves.
>PLoS ONE 11(7): e0158747.

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  • IgG Antibody thumbnail image 1
  • IgG Antibody thumbnail image 2
  • IgG Antibody thumbnail image 3
  • IgG Antibody thumbnail image 4
  • IgG Antibody thumbnail image 5
  • IgG Antibody thumbnail image 6
  • Sheep anti Bovine IgG:Biotin
  • Sheep anti Bovine IgG
  • Sheep anti Bovine IgG:Alk. Phos.
  • Sheep anti Bovine IgG:FITC
  • Sheep anti Bovine IgG:HRP
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  • Product Type
    Polyclonal Antibody
  • Isotype
    Polyclonal IgG
5 Formats Available
    Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
    AAI23ABE, WBdatasheet pdfdatasheet pdf0.5 mg
    AAI23AB
    AAI23BE, WBdatasheet pdfdatasheet pdf1 mg
    AAI23B
    AAI23E, IDdatasheet pdfdatasheet pdf1 mg
    AAI23
    AAI23FC, Fdatasheet pdfdatasheet pdf1 mg
    AAI23F
    AAI23PE, WBdatasheet pdfdatasheet pdf1 mg
    AAI23P
    Summary
    Secondary Antibodies
    Negative Isotype Controls
    Useful Reagents
    Positive Controls
    Histology Controls
    More Images
    References
    Reviews
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    • Sheep anti Bovine IgG polyclonal antibody recognizes bovine IgG and shows no cross - reactivity with other bovine immunoglobulin classes in immunoelectrophoresis. This polyclonal antibody has not been cross adsorbed and may therefore react with IgG from other species

      Sheep anti Bovine IgG has been usefully employed for the detection of antigen specific antibody reactivity in cattle by ELISA (Vrieling et al. 2013).
    • Intended Use
    • Target Species
      Bovine
    • Product Form
      Purified IgG fraction conjugated to Biotin - liquid
      Purified IgG - liquid
      Purified IgG conjugated to Alkaline Phosphatase - liquid
      Purified IgG fraction conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
      Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
    • Reconstitution
    • Preparation
    • Antiserum Preparation
      Antisera to bovine IgG were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG prepared by affinity chromatography.
    • Preservative Stabilisers
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      0.09% Sodium Azide (NaN3)
      0.09%Sodium Azide
      0.1% Proclin™ 300
    • Immunogen
      Purified bovine IgG.
    • Purity
    • Approx. Protein Concentrations
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 0.5mg/ml
      IgG concentration 1.0 mg/ml
      IgG concentration 1.0 mg/ml
    • Reagents In The Kit
    • Preparing The Antibody
    • Test Principle
    • Buffer Solution
      Phosphate buffered saline
      Phosphate buffered saline
      50mM HEPES, 0.1M NaCl, 1mM MgCl2, 0.1mM ZnCl2
      Phosphate buffered saline
      Phosphate buffered saline
    • Storage
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC.
      DO NOT FREEZE.

      This product should be stored undiluted.
      Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
      Store at +4oC. DO NOT FREEZE.
      This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
    • Shelf Life
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
      12 months from date of despatch.
    • Acknowledgements
      Proclin™ 300 is a trademark of The Dow Chemical Company (“Dow”) or an affiliated company of Dow.
    • Regulatory
      For research purposes only
    • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

    • Application NameYesNoMin DilutionMax Dilution
      ELISA1:100001:100000
      Western Blotting1:100001:100000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/1001/10,000
      Immunodiffusion
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/50001/50000
      Western Blotting1/50001/50000
    • Application NameYesNoMin DilutionMax Dilution
      Flow Cytometry
      Immunohistology - Frozen1/2001/2,000
    • Application NameYesNoMin DilutionMax Dilution
      ELISA1/10,0001/100,000
      Western Blotting

    • Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using the appropriate negative/positive controls.
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Technical Advice
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • Recommended Protocol
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • ELISA
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Immunohistology
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Histology Positive Control Tissue
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Immunofluorescence
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Western Blotting
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use
    • Instructions For Use

    Additional IgG Antibody Formats

    Formats Applications Sizes available
    IgG Antibody : Purified E, ID 1 mg
    IgG Antibody : Alk. Phos. E, WB 0.5 mg
    IgG Antibody : Biotin E, WB 1 mg
    IgG Antibody : FITC C, F 1 mg
    IgG Antibody : HRP E, WB 1 mg
    • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

    Recommended Secondary Antibody

      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Streptavidin:APCSTAR119100 TestsF
      STAR119
      DescriptionProduct CodePack SizeApplicationsList PriceQuantity
      Rabbit anti Sheep IgG (H/L):Biotin5184-23041.5 mlC, E, F, WB
      5184-2304
      Donkey anti Sheep/Goat IgG:DyLight®488STAR88D488GA0.1 mgF, IF
      STAR88D488GA
      Donkey anti Sheep/Goat IgG:DyLight®549STAR88D549GA0.1 mgF, IF
      STAR88D549GA
      Donkey anti Sheep/Goat IgG:DyLight®649STAR88D649GA0.1 mgF, IF
      STAR88D649GA
      Donkey anti Sheep/Goat IgG:FITCSTAR88F1 mgC
      STAR88F
      Donkey anti Sheep/Goat IgG:HRPSTAR88P1 mlC, E, P, WB
      STAR88P

      Recommended Negative Isotype Control

        Useful Reagents

          DescriptionProduct CodePack SizeApplicationsList PriceQuantity
          AbGUARD® HRP Stabilizer PlusBUF052A100 mlC, E, P, WB
          BUF052A
          TMB Signal+BUF054A100 mlE
          BUF054A
          TMB CoreBUF056A100 mlE
          BUF056A
          TMB Core PrestainedBUF057A100 mlE
          BUF057A
          TMB Core+BUF062A100 mlE
          BUF062A
          AbGUARD® HRP Stabilizer PlusBUF052C1000 mlC, E, P, WB
          BUF052C
          AbGUARD® HRP Stabilizer PlusBUF052B500 mlC, E, P, WB
          BUF052B

          Recommended Positive Controls

            Histology Controls

              References

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