Hyaluronidase catalyzes the depolymerization of mucopolysaccharides, hyaluronic acid, and the chondroitin sulfates A and C. The enzyme is widely distributed in animal tissues but is found in great concentrations in the bovine and ovine testes.
Intended Use
Target Species
Bovine
Product Form
Purified enzyme from bovine testes - liquid
Reconstitution
Preparation
Preservative Stabilisers
None present
Activity
315 U/mg protein 1 unit is defined as the amount of enzyme that liberates one micromole of N-acetylglucosamine per minute at 37ºC and pH 4.0. EC 3.2.1.36
Purity
Approx. Protein Concentrations
Total protein concentration 0.2 g/ml
Molecular Weight
MW: 55 kD
Reagents In The Kit
Preparing The Antibody
Test Principle
Buffer Solution
Storage
Store at -20oC only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name
Yes
No
Min Dilution
Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.