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Collagen I

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Native Bovine Collagen I

Product Type
Purified Protein
Specificity
Collagen I

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Product Code Applications Pack Size List Price Your Price Qty
2150-0515
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 10 mg loader
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Collagen type I 90%
Collagen type III 10%
Non-collagenous proteins <0.5%

Target Species
Bovine
Product Form
Purified protein from calf tendon - lyophilized
Reconstitution
Use 0.5M acetic acid, pH 3.0. Dispersed collagen have to be thoroughly homogenized in a Potter after addition of the solute and left overnight in the coldroom to dissolve.
Dissolved collagen retains immunologic properties of native collagen type I. Thermal denaturation (5 minutes at 100ºC) converts dissolved collagen to gelatin.
Preparation
Bovine collagen type I was isolated from calf tendons. Isolation steps included extraction into dilute acetic acid after pepsin treatment, followed by multiple steps of differential salt precipitation and ion-exchange chromatography.
Buffer Solution
Essentially salt free
Preservative Stabilisers
None present
Protein Molecular Weight
~300 kDa
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

UniProt
P02465
P02453
Entrez Gene
COL1A1
COL1A2
GO Terms
GO:0005201 extracellular matrix structural constituent

2150-0515

150826 157466 160443 165247

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