Flow cytometric staining of bovine peripheral blood lymphocytes. A. Mouse IgG1 negative control (MCA928) followed by Goat anti Mouse IgG1:PE (STAR132PE). B. Mouse anti Bovine CD4 antibody, clone CACT138A (MCA6081) followed by Goat anti Mouse IgG1:PE secondary antibody (STAR132PE). Both the Mouse IgG1 negative control and Mouse anti Bovine CD4 are plotted against bovine CD8 as recognized by FITC conjugated Mouse anti Bovine CD8 antibody, clone CC63 (MCA837F).
Mouse anti Bovine CD4, clone CACT138A, recognizes bovine CD4. CD4 regulates T cell activation and acts as an accessory protein for MHC II and TCR interaction. It is a marker for bovine T helper cells. In cattle CD4+ T cells can also be found in mediastinal haemolymph nodes, lymph nodes and parafollicular lymphatic tissue (Galeotti et al. 1993). Cattle infected with viral diarrhea virus had depleted numbers of CD4+ cells (Endsley et al. 2002).
N.B. Antibody reactivity and working conditions may vary between species.
Concentrated tissue culture supernatant - liquid
Concentrated tissue culture supernatant clarified by filtration through a 0.2 micrometer filter
0.09% Sodium Azide (NaN3)
ConA activated bovine lymphocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Reagents In The Kit
Preparing The Antibody
Serum free tissue culture medium containing proprietary protein free supplement
Store at +4oC. DO NOT FREEZE. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of despatch
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells in 100ul